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| | TRYPSIN-EDTA SOLUTION Basic information |
| Product Name: | TRYPSIN-EDTA SOLUTION | | Synonyms: | TRYPSIN-EDTA SOLUTION;0.05% Trypsin Cell Digestion Solution;0.25% Trypsin Cell Digestion Solution;0.25% Trypsin Cell Dissociation Solution (without EDTA);0.25% Trypsin Cell Dissociation Solution (with Phenol Red);Trypsin Cell Digestion Solution (0.05% Trypsin);Trypsin Cell Digestion Solution (0.25% Trypsin, without EDTA);Trypsin Cell Digestion Solution (0.25% Trypsin) | | CAS: | | | MF: | | | MW: | 0 | | EINECS: | | | Product Categories: | | | Mol File: | Mol File | ![TRYPSIN-EDTA SOLUTION Structure]() |
| | TRYPSIN-EDTA SOLUTION Chemical Properties |
| storage temp. | -20°C | | form | solution | | PH | 7.0-9.0 | | biological source | Porcine pancreas |
| WGK Germany | WGK 1 | | Storage Class | 12 - Non Combustible Liquids |
| | TRYPSIN-EDTA SOLUTION Usage And Synthesis |
| Uses | The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. It can be used with endothelial cell cultures. | | Biochem/physiol Actions | Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity. Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin. |
| | TRYPSIN-EDTA SOLUTION Preparation Products And Raw materials |
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