N-[(1S)-1-(氨基羰基)-4 -[(2 - 氯-1-亚氨基乙基氨基]丁基]-苯甲酰胺盐酸盐 CAS#: 1373232-26-8

N-[(1S)-1-(氨基羰基)-4 -[(2 - 氯-1-亚氨基乙基氨基]丁基]-苯甲酰胺盐酸盐

生物活性靶点体外研究体内研究用途与合成方法 MSDS N-[(1S)-1-(氨基羰基)-4 -[(2 - 氯-1-亚氨基乙基氨基]丁基]-苯甲酰胺盐酸盐价格(试剂级) 上下游产品信息

中文名称	N-[(1S)-1-(氨基羰基)-4 -[(2 - 氯-1-亚氨基乙基氨基]丁基]-苯甲酰胺盐酸盐
中文同义词	N-[(1S)-1-(氨基羰基)-4 -[(2 - 氯-1-亚氨基乙基氨基]丁基]-苯甲酰胺盐酸盐;CL-M(盐酸盐);化合物CL-AMIDINE HYDROCHLORIDE;化合物CL-AMIDINE HYDROCHLORIDE,10 MM DMSO 溶液;(S)-N-(1-氨基-5-(2-氯乙脒)-1-氧代戊烷-2-基)苯甲酰胺盐酸盐;Cl-amidine hydrochloride试剂;Cl-amidine hydrochloride试剂
英文名称	N-[(1S)-1-(Aminocarbonyl)-4-[(2-chloro-1-iminoethyl)amino]butyl]-benzamide hydrochloride
英文同义词	N-[(1S)-1-(Aminocarbonyl)-4-[(2-chloro-1-iminoethyl)amino]butyl]-benzamide hydrochloride;Cl-Amidine (hydrochloride) - Sold under license from the University of South Carolina under U.S. Patent No. 7,964,363;Cl-Amidine (hydrochloride);Cl amidine hydrochloride,miRNA-16,Protein Arginine Deiminase,Cl-amidine,inhibit,MicroRNA,Peptidylarginine Deiminase,Clamidine hydrochloride,Inhibitor,miRNA,microRNA-16,colitis,sepsis,Apoptosis,miR-16;Cl-amidine hydrochloride, 10 mM in DMSO;(S)-N-(1-Amino-5-(2-chloroacetimidamido)-1-oxopentan-2-yl)benzamide hydrochloride
CAS号	1373232-26-8
分子式	C14H20Cl2N4O2
分子量	347.2402
EINECS号
相关类别	试剂盒-细胞分析试剂盒
Mol文件	1373232-26-8.mol
结构式

N-[(1S)-1-(氨基羰基)-4 -[(2 - 氯-1-亚氨基乙基氨基]丁基]-苯甲酰胺盐酸盐性质

储存条件	Store at -20°C
溶解度	DMF：14mg/mL； DMSO：50mg/mL；乙醇：20mg/mL； PBS（pH 7.2）：3 mg/mL
形态	结晶固体
颜色	白色至浅黄色

N-[(1S)-1-(氨基羰基)-4 -[(2 - 氯-1-亚氨基乙基氨基]丁基]-苯甲酰胺盐酸盐用途与合成方法

生物活性

Cl-amidine hydrochloride 是口服有效的 PAD 抑制剂，其对 PAD1、PAD3 和 PAD4 的 IC50 值分别为0.8 μM、 6.2 μM 和 5.9 μM。Cl-amidine hydrochloride 可诱导癌细胞的凋亡。Cl-amidine hydrochloride 可诱导 miR-16，引起细胞周期阻滞。Cl-Amidine hydrochloride 可阻断组蛋白 3 瓜氨酸化和中性粒细胞胞外陷阱的形成，并提高败血症小鼠的存活率。

靶点

IC50: 0.8 μM (PAD1), 5.9 μM (PAD4), 6.2 μM (PAD3).

体外研究

Cl-amidine is a bioavailable haloacetamidine-based compound that inhibits all the active PAD isozymes with near equal potency (k _inact /K _I =13,000 M _-1 •min-1 for PAD4).
Cl-amidine (0, 5, 10, 15, 20, 25, 50 μg/mL, 24 hours) induces apoptosis in TK6 lymphoblastoid cells and HT29 colon cancer cells in a dose-dependent manner. Interestingly, the colon cancer cell line (HT29) is relatively resistant to apoptosis caused by Cl-amidine.
Cl-Amidine prevents histone 3 citrullination and neutrophil extracellular trap formation, and improves survival in a murine sepsis model.

Apoptosis Analysis.

Cell Line:	TK6 lymphoblastoid cells and HT29 colon cancer cells.
Concentration:	0, 5, 10, 15, 20, 25, 50 μg/mL.
Incubation Time:	24 h.
Result:	Induced apoptosis dose-dependently.

体内研究

Cl-amidine (75 mg/kg, ip once daily) suppresses and treats DSS-induced colitis in mice.
Cl-amidine (5, 25, 75 mg/kg, oral gavage, once daily) leads to significant reductions in the histology scores dose-dependently.

Animal Model:	C57BL/6 mice (8-12 wk old, DSS mouse model of colitis).
Dosage:	75 mg/kg.
Administration:	IP once daily.
Result:	Suppressed PAD activity, protein citrullination, and PAD levels in the colon in vivo.

Animal Model:	C57BL/6 mice (8-12 wk old, DSS mouse model of colitis).
Dosage:	5, 25, 75 mg/kg.
Administration:	Oral gavage once daily.
Result:	Led to significant reductions in the histology scores.

安全信息

MSDS信息

N-[(1S)-1-(氨基羰基)-4 -[(2 - 氯-1-亚氨基乙基氨基]丁基]-苯甲酰胺盐酸盐价格(试剂级)

更新日期	产品编号	产品名称	CAS号	包装	价格
2025/12/22	HY-100574A	N-[(1S)-1-(氨基羰基)-4 -[(2 - 氯-1-亚氨基乙基氨基]丁基]-苯甲酰胺盐酸盐 Cl-amidine hydrochloride	1373232-26-8	1 mg	502元
2025/12/22	HY-100574A	N-[(1S)-1-(氨基羰基)-4 -[(2 - 氯-1-亚氨基乙基氨基]丁基]-苯甲酰胺盐酸盐 Cl-amidine hydrochloride	1373232-26-8	10mM * 1mLin DMSO	917元

N-[(1S)-1-(氨基羰基)-4 -[(2 - 氯-1-亚氨基乙基氨基]丁基]-苯甲酰胺盐酸盐