ML334
| 中文名称 | ML334 |
|---|---|
| 中文同义词 | 化合物 T12078;(1S,2R)-2-((S)-1-((1,3-二氧代异吲哚啉-2-基)甲基)-1,2,3,4-四氢异喹啉-2-羰基)环己烷-1-甲酸;化合物 ML334;化合物ML334,10 MM DMSO 溶液 |
| 英文名称 | ML334 |
| 英文同义词 | ML334;(1S,2R)-2-[[(1S)-1-[(1,3-Dihydro-1,3-dioxo-2H-isoindol-2-yl)methyl]-3,4-dihydro-2(1H)-isoquinolinyl]carbonyl]cyclohexanecarboxylic acid;(1S,2R)-2-[(1S)-1-[(1,3-dioxo-2,3-dihydro-1H-isoindol-2-yl)methyl]-1,2,3,4-tetrahydroisoquinolin-2-carbonyl]cyclohexane-1-carboxylic acid;LH601A;Cyclohexanecarboxylic acid, 2-[[(1S)-1-[(1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)methyl]-3,4-dihydro-2(1H)-isoquinolinyl]carbonyl]-, (1S,2R)-;ML334,ML-334;ML334, 10 mM in DMSO;Keap1-Nrf2 Interaction Probe, ML334 |
| CAS号 | 1432500-66-7 |
| 分子式 | C26H26N2O5 |
| 分子量 | 446.49 |
| EINECS号 | |
| 相关类别 | |
| Mol文件 | 1432500-66-7.mol |
| 结构式 |  |
ML334 性质
| 沸点 | 693.1±55.0 °C(Predicted) |
|---|---|
| 密度 | 1.354±0.06 g/cm3(Predicted) |
| 储存条件 | Store at 4°C, stored under nitrogen |
| 溶解度 | 二甲基亚砜：40mg/mL |
| 酸度系数(pKa) | 4.69±0.44(Predicted) |
| 形态 | 固体 |
| 颜色 | 白色至米白色 |
| InChIKey | LNENLABLFGGAFF-BHIFYINESA-N |
| SMILES | N4(C(=O)c5c(cccc5)C4=O)C[C@H]1N(CCc3c1cccc3)C(=O)[C@H]2[C@H](CCCC2)C(=O)O |
ML334 (LH601A) 是一种有效的，细胞可渗透的 NRF2 激活剂，通过抑制 Keap1-NRF2 蛋白质相互作用来发挥作用。ML334 以 Kd 值为 1μM 来结合 Keap1 Kelch 域。ML334 刺激 NRF2 表达和核易位并诱导抗氧化反应元件 (ARE) 活性。
Ki: 1 μM (Keap1)
ML334 (LH601A; 50-100 µM; 6-16 hours; HEK293 cells) treatment increases NQO1 and TRX1 mRNAs between 2- and 3-fold at both 6 and 16 h. And also enhances HO-1 mRNA expression between 4- and 7-fold at 6 h, with lesser fold-changes at 16 h.
ML334 (LH601A; 50-100 µM; 16 hours; HEK293 cells) treatment induces HO-1 and TRX1 proteins in HEK293 cells.
ML334 (LH601A) stimulates NRF2 expression and nuclear translocation in HEK293 cells.
RT-PCR
| Cell Line: | HEK293 cells |
| Concentration: | 50 μM or 100 µM |
| Incubation Time: | 6 hours, 16 hours |
| Result: | Increased NQO1 and TRX1 mRNAs between 2- and 3-fold at both 6 and 16 h. Enhanced HO-1 mRNA expression between 4- and 7-fold at 6 h. |
Western Blot Analysis
| Cell Line: | HEK293 cells |
| Concentration: | 50 μM or 100 µM |
| Incubation Time: | 16 hours |
| Result: | Enhanced HO-1 and TRX1 protein expression at 16 h. |
安全信息
| WGK Germany | WGK 3 |
|---|---|
| 存储类别 | 11 - 可燃固体 |
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2025/12/22 | HY-110258 | ML334 ML334 | 1432500-66-7 | 1mg | 681元 |
| 2025/12/22 | HY-110258 | ML334 ML334 | 1432500-66-7 | 5mg | 1500元 |