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| | Cy5 Tetrazine Basic information |
| | Cy5 Tetrazine Chemical Properties |
| solubility | Water, DMSO, DMF | | Appearance | Blue solid |
| | Cy5 Tetrazine Usage And Synthesis |
| Description | Terazine-activated Cy5 dye reacts with TCO-containing compounds via a Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.Cy5 Tetrazine is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.This is sulfonated dye is also known as sulfo-Cyanine5. | | Abs/Em Maxima | 649/671 nm | | Extinction Coefficient | 250,000 | | Microscopy Laser Line | 633 or 635 nm | | Flow Cytometry Laser Line | 633 or 635 nm | | Spectrally Similar Dyes | Alexa Fluor® 647, Atto™ 647, CF® 647 Dye, DyLight® 649 |
| | Cy5 Tetrazine Preparation Products And Raw materials |
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