| Company Name: |
Sigma-Aldrich |
| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
|
| | BCIP/NBT Basic information |
| Product Name: | BCIP/NBT | | Synonyms: | BCIP/NBT;1-STEP NBT/BCIP SOLUTION;5-BROMO-4-CHLORO-3-INDOLYLPHOSPHATE/IODONITROTETRAZOLIUM CHLORIDE;5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE/P-IODONITROTETRAZOLIUM;5-BROMO, 4-CHLORO, 3-INDOLYPHOSPHATE/IODONITROTETRAZOLIUM;BCIP/NBT LIQUID SUBSTRATE SYSTEM;BCIP/NBT PLUS;BCIP/NBT STABILIZED CHROMOGEN | | CAS: | | | MF: | C48H34BrClN11O10P | | MW: | 1071.18 | | EINECS: | | | Product Categories: | | | Mol File: | Mol File |  |
| | BCIP/NBT Chemical Properties |
| Fp | 67 °C | | storage temp. | -20°C | | form | tablet | | color | dark purple | | Water Solubility | water: 10mL, clear, yellow |
| Hazard Codes | Xi | | Risk Statements | 36/37/38 | | Safety Statements | 26 | | WGK Germany | 2 | | Storage Class | 12 - Non Combustible Liquids | | Hazard Classifications | Skin Sens. 1 |
| | BCIP/NBT Usage And Synthesis |
| Uses | A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures. | | Uses | SIGMAFAST BCIP/NBT has been used as a substrate
- for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting
- for alkaline phosphatase in the histochemical staining of primary osteoblasts
- for alkaline phosphatase staining in bone marrow stromal cells
| | General Description | BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.
Appearance prior to use: light yellow color;
After color development: dark purple |
| | BCIP/NBT Preparation Products And Raw materials |
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