去氧鬼臼脂素
| 中文名称 | 去氧鬼臼脂素 |
|---|---|
| 中文同义词 | 去氧鬼臼脂素;去氧鬼臼毒素,去氧鬼臼脂素;脱氧鬼臼毒素,脱氧鬼臼脂素;去氧鬼臼毒素标准品;去氧鬼臼毒素对照品;脱氧鬼臼毒素;鬼臼毒素杂质5;(5R,5AR,8AR)-5-(3,4,5-三甲氧基苯基)-5,8,8A,9-四氢呋喃并[3',4':6,7]萘并[2,3-D][1,3]二氧戊环-6(5AH)-酮 |
| 英文名称 | Desoxypodophyllotoxin |
| 英文同义词 | Desoxypodophyllotoxin;anthric;Anthricin/Deoxypodophyllotoxin;4-Deoxypodophyllotoxin;Furo[3',4':6,7]naphtho[2,3-D]-1,3-dioxol-6(5ah)-one, 5,8,8A,9-tetrahydro-5-(3,4,5-trimethoxyphenyl)-,(5R,5ar,8ar)-;Nsc403148;Deoxypodophyllotoxin, deoxypodophyllotoxin;Podophyllotoxin 9-Desoxy Impurity |
| CAS号 | 19186-35-7 |
| 分子式 | C22H22O7 |
| 分子量 | 398.41 |
| EINECS号 | |
| 相关类别 | 对照品;标准品;植物提取物;标准品 -对照药材;木脂素 |
| Mol文件 | 19186-35-7.mol |
| 结构式 |  |
去氧鬼臼脂素 性质
| 储存条件 | Store at 2-8°C |
|---|---|
| 溶解度 | 溶于氯仿、二氯甲烷、乙酸乙酯、DMSO、丙酮等。 |
| 密度 | 1.304±0.06 g/cm3(Predicted) |
| 形态 | 粉末 |
| 沸点 | 564.5±50.0 °C(Predicted) |
| 熔点 | 168 °C |
| 颜色 | 白色至米白色 |
| 稳定性 | 吸湿性 |
| InChI | InChI=1S/C22H22O7/c1-24-17-6-12(7-18(25-2)21(17)26-3)19-14-8-16-15(28-10-29-16)5-11(14)4-13-9-27-22(23)20(13)19/h5-8,13,19-20H,4,9-10H2,1-3H3/t13-,19+,20-/m0/s1 |
| InChIKey | ZGLXUQQMLLIKAN-SVIJTADQSA-N |
| SMILES | O1C2=CC3=C(C=C2OC1)[C@@H](C1=CC(OC)=C(OC)C(OC)=C1)[C@@]1([H])C(=O)OC[C@]1([H])C3 |
Deoxypodophyllotoxin (25-75 nM; 6-48 hours) increases the percentage of early apoptotic cell population from 2.05 to 5.62 and 18.49% for 24 h and 48 h, respectively. Deoxypodophyllotoxin (25-75 nM; 6-48 hours) treats SGC-7901 cells arrested in G2/M phase in time- and dose- dependent manners. Deoxypodophyllotoxin (25-75 nM; 6-48 hours) results in a remarkably time- and dose-dependent decrease in Cdc2 and Cdc25C expression levels and increases cyclin B1 within 6h, decreases PARP, Bcl-2 and caspase-3 activity.
Apoptosis Analysis
| Cell Line: | SGC-7901 cells |
| Concentration: | 25, 50, 75 nM |
| Incubation Time: | 6, 12, 24, 48 hours |
| Result: | Induced apoptosis in SGC-7901 Cells. |
Cell Cycle Analysis
| Cell Line: | SGC-7901 cells |
| Concentration: | 25, 50, 75 nM |
| Incubation Time: | 6, 12, 24, 48 hours |
| Result: | Induced G2/M cell cycle arrest in SGC-7901 Cells |
Western Blot Analysis
| Cell Line: | SGC-7901 cells |
| Concentration: | 25, 50, 75 nM |
| Incubation Time: | 6, 12, 24, 48 hours |
| Result: | Altered the expression of cyclin B1, Cdc2,Cdc25C, p-PARP, Bcl-2 and p-caspase-3 proteins. |
Deoxypodophyllotoxin (intravenously injected; 5, 10, and 20 mg/kg; 3 times a week; 28 days) suppresses the tumors in a dose-dependent manner, the growth of tumors is inhibited by 22.19%, 47.91% and 50.93% with DPT at 5, 10 and 20 mg/kg, respectively.
| Animal Model: | Xenograft model of gastric cancer in nude mice with SGC-7901 cells |
| Dosage: | 5, 10, and 20 mg/kg |
| Administration: | Intravenously injected; 5, 10, and 20 mg/kg; 3 times a week; 28 days |
| Result: | Inhibited the growth of gastric cancer tumors. |