| Company Name: |
Sigma-Aldrich |
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021-61415566 800-8193336 |
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orderCN@merckgroup.com |
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| | Yo-Pro 1 Basic information |
| Product Name: | Yo-Pro 1 | | Synonyms: | Quinolinium, 4-((3-methyl-2(3H)-benzoxazolylidene)methyl)-1-(3-(trimethylammonio)propyl)-, diiodide;Yo-pro 1
iodide;Oxazole yellow >=98% (HPLC);Oxazole yellow, 1mM in DMSO;Oxazole yellow, ≥99%;YO-PRO-1 (oxazole yellow);3H-Indolium, 2-[2-[3-[2-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)ethylidene]-1-cyclohexen-1-yl]ethenyl]-3,3-dimethyl-1-[6-oxo-6-(2-propyn-1-ylamino)hexyl]-, chloride (1:1) (ACI);Yo | | CAS: | 152068-09-2 | | MF: | C24H29IN3O+ | | MW: | 502.42 | | EINECS: | | | Product Categories: | Cyanine | | Mol File: | 152068-09-2.mol |  |
| | Yo-Pro 1 Chemical Properties |
| Melting point | >250 ℃ | | storage temp. | 2-8°C | | solubility | H2O: soluble5, clear (warmed) | | form | powder | | color | yellow to orange-brown | | Appearance | Green solution | | biological source | r at blood | | Water Solubility | H2O: 5, clear (warmed) | | λmax | 491 nm | | ex/em | 491/509 nm | | ε(extinction coefficient) | 52000 cm−1M−1 | | Φ(quantum yield) | 0.44 | | Biological Applications | Nucleic acid hybridization; nucleic acid sequencing; detecting nucleic acids,cells,bacteria,immunodeficiency virus,microorganisms; counting embryoblasts,viruses; apoptosis assay; white blood cell functional assay | | InChI | 1S/C24H29N3O.2HI/c1-25-22-12-7-8-13-23(22)28-24(25)18-19-14-16-26(15-9-17-27(2,3)4)21-11-6-5-10-20(19)21;;/h5-8,10-14,16,18H,9,15,17H2,1-4H3;2*1H/q+2;;/p-2 | | InChIKey | ULHRKLSNHXXJLO-UHFFFAOYSA-L | | SMILES | CN1C2=CC=CC=C2OC1=CC3=CC=[N+](CCC[N+](C)(C)C)C4=C3C=CC=C4 |
| RIDADR | UN 2811 6.1 / PGIII | | WGK Germany | WGK 3 | | Storage Class | 6.1C - Combustible acute toxic Cat.3
toxic compounds or compounds which causing chronic effects | | Hazard Classifications | Acute Tox. 3 Oral
Eye Irrit. 2
Skin Irrit. 2 |
| | Yo-Pro 1 Usage And Synthesis |
| Description | YO-TAP-1 (Oxazole Yellow Monomer, also known as YO-PRO®-1) is a green fluorescent carbocyanine monomeric dye. YO-TAP-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a significant fluorescence enhancement upon binding to dsDNA.
YO-TAP-1 has a bright fluorescence signal and low background. The dye is useful for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in microscopy. It may be used for monitoring of cell viability and apoptotic and dead cell detection in culture. | | Chemical Properties | Appearance: Green solution
ex/em: 491/509 nm
Extinction coefficient (ε): 52000 cm1M1
Quantum yield (Φ): 0.44 | | Uses | Hybridisation and cloning studies | | Definition | ChEBI: An unsymmetrical C1 cyanine dye having 1,3-benzoxazol-2-yl and quinolinium-4-yl substituents. | | Biochem/physiol Actions | Oxazole yellow is a fluorescent cyanine dye that binds to DNA and is used to detect apoptotic cells. Oxazole yellow does not enter live cells. However, during apoptosis the cytoplasmic membrane becomes slightly permeable, allowing entry of the dye. Oxazole yellow is often used along with propidium iodide (catalog no. P4170), a DNA dead cell stain that does not enter either live or apoptotic cells. The λEx/λEm of Oxazole yellow is 491/509. | | storage | -20°C (protect from light) | | Abs/Em Maxima | 491/508 nm |
| | Yo-Pro 1 Preparation Products And Raw materials |
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