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| | Cyanine5 Biotin Basic information |
| Product Name: | Cyanine5 Biotin | | Synonyms: | Cyanine5 Biotin;Cy5 Biotin | | CAS: | | | MF: | | | MW: | 0 | | EINECS: | | | Product Categories: | | | Mol File: | Mol File | ![Cyanine5 Biotin Structure]() |
| | Cyanine5 Biotin Chemical Properties |
| solubility | Water, DMSO, DMF, MeOH | | Appearance | Blue solid |
| | Cyanine5 Biotin Usage And Synthesis |
| Description | Cy5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. Cy5 Biotin is a water soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin. | | Abs/Em Maxima | 649/671 nm | | Extinction Coefficient | 250,000 | | Microscopy Laser Line | 633 or 635 nm | | Flow Cytometry Laser Line | 633 or 635 nm | | Spectrally Similar Dyes | Alexa Fluor® 647, CF™ 647 Dye, DyLight™649 |
| | Cyanine5 Biotin Preparation Products And Raw materials |
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