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| | Glucose Colorimetric Assay Kit Basic information |
| | Glucose Colorimetric Assay Kit Chemical Properties |
| | Glucose Colorimetric Assay Kit Usage And Synthesis |
| Description | Glucose, a monosaccharide (or simple sugar), is the most important carbohydrate in biology. Transported via the blood stream, it is the primary source of energy for the body’s cells. Glucose level is tightly regulated in the human body. Failure to maintain blood glucose in the normal range leads to conditions of persistently high (hyperglycemia) or low (hypoglycemia) blood sugar. Diabetes mellitus, characterized by persistent hyperglycemia, is the most prominent disease related to failure of blood sugar regulation. Cayman’s Glucose Colorimetric Assay Kit provides a simple, reproducible, and sensitive tool for assaying glucose in plasma, serum, and urine. The glucose assay uses the glucose oxidase-peroxide reaction for the determination of glucose concentrations. In this assay, glucose is oxidized to δ-gluconolactone with concomitant reduction of the flavin adenine dinucleotide (FAD)-dependent enzyme glucose oxidase. The reduced form of glucose oxidase is regenerated to its oxidized form by molecular oxygen to produce hydrogen peroxide. Finally, with horseradish peroxidase as a catalyst, hydrogen peroxide reacts with 3,5-dichloro-2-hydroxybenzenesulfonic acid and 4-aminoantipyrine to generate a pink dye with an optimal absorption at 514 nm. | | References | [1] JACOB E HOOD Rodney D A Sumith Yesudasan. Glucose Concentration Affects Fibrin Clot Structure and Morphology as Evidenced by Fluorescence Imaging and Molecular Simulations.[J]. Clinical and Applied Thrombosis/Hemostasis, 2018, 24 9_suppl: 104S-116S. DOI: 10.1177/1076029618792304 [2] SONAM KUMARI. MUC13 contributes to rewiring of glucose metabolism in pancreatic cancer.[J]. Oncogenesis, 2018: 19. DOI: 10.1038/s41389-018-0031-0 [3] MARKUS MüHLEMANN . Altered pancreatic islet morphology and function in SGLT1 knockout mice on a glucose-deficient, fat-enriched diet[J]. Molecular Metabolism, 2018, 13: Pages 67-76. DOI: 10.1016/j.molmet.2018.05.011 [4] REBECCA L SCALZO. Glucagon-like peptide-1 receptor antagonism impairs basal exercise capacity and vascular adaptation to aerobic exercise training in rats.[J]. Physiological Reports, 2018, 6 13: e13754. DOI: 10.14814/phy2.13754 [5] PAUL R LEE. Protease-activated receptor-1 activation by granzyme B causes neurotoxicity that is augmented by interleukin-1β.[J]. Journal of Neuroinflammation, 2017: 131. DOI: 10.1186/s12974-017-0901-y [6] WILLIAM W FRENCH. A High-Protein Diet Reduces Weight Gain, Decreases Food Intake, Decreases Liver Fat Deposition, and Improves Markers of Muscle Metabolism in Obese Zucker Rats.[J]. Nutrients, 2017, 9 6. DOI: 10.3390/nu9060587 [7] JESSE BERTINATO. Moderately Low Magnesium Intake Impairs Growth of Lean Body Mass in Obese-Prone and Obese-Resistant Rats Fed a High-Energy Diet.[J]. Nutrients, 2016, 8 5. DOI: 10.3390/nu8050253 [8] KELLY L. HARRIS. Western diet enhances benzo(a)pyrene-induced colon tumorigenesis in a polyposis in rat coli (PIRC) rat model of colon cancer[J]. Oncotarget, 2016, 7 1: 28947-28960. DOI: 10.18632/oncotarget.7901 [9] HUNG-JEN LU. Ruscogenin ameliorates experimental nonalcoholic steatohepatitis via suppressing lipogenesis and inflammatory pathway.[J]. BioMed Research International, 2014: 652680. DOI: 10.1155/2014/652680 [10] THING-FONG TZENG. Zerumbone, a Natural Cyclic Sesquiterpene of Zingiber zerumbet Smith, Attenuates Nonalcoholic Fatty Liver Disease in Hamsters Fed on High-Fat Diet.[J]. Evidence-based Complementary and Alternative Medicine, 2013, 2013: 303061. DOI: 10.1155/2013/303061 |
| | Glucose Colorimetric Assay Kit Preparation Products And Raw materials |
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