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| | Bicinchoninic Acid Disodium Salt Basic information |
| Product Name: | Bicinchoninic Acid Disodium Salt | | Synonyms: | SodiumBicinchoninate;BCA DISODIUM SALT;BCA;BICINCHONINIC ACID DISODIUM SALT;BICINCHONINIC ACID DISODIUM SALT HYDRATE;DI-SODIUM 2,2'-BICINCHONINATE;DISODIUM 2,2'-BIQUINOLINE-4,4'-DICARBOXYLATE;2,2'-BICINCHONINIC ACID DISODIUM SALT | | CAS: | 979-88-4 | | MF: | C20H13N2NaO4 | | MW: | 368.32 | | EINECS: | 629-761-2 | | Product Categories: | marker | | Mol File: | 979-88-4.mol |  |
| | Bicinchoninic Acid Disodium Salt Chemical Properties |
| Melting point | 300℃ | | storage temp. | Inert atmosphere,2-8°C | | solubility | 0.5% sodium bicarbonate: soluble50mg/mL | | form | Powder/Chunks | | color | white to light yellow | | Water Solubility | Soluble in water. | | Stability: | Hygrscopic | | InChI | InChI=1S/C20H12N2O4.Na.H/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18;;/h1-10H,(H,23,24)(H,25,26);; | | InChIKey | AUPXFICLXPLHBB-UHFFFAOYSA-L | | SMILES | C(C1=CC(C2=NC3=CC=CC=C3C(C(=O)O)=C2)=NC2=CC=CC=C12)(=O)O.[NaH] | | CAS DataBase Reference | 979-88-4(CAS DataBase Reference) | | EPA Substance Registry System | [2,2'-Biquinoline]-4,4'-dicarboxylic acid, disodium salt (979-88-4) |
| | Bicinchoninic Acid Disodium Salt Usage And Synthesis |
| Description | 2,2’-Biquinoline-4,4’-dicarboxylic acid (BCA) is a colorimetric reagent for the detection of copper (Cu+). BCA and Cu+ form a stable 2:1 complex that has an absorbance maximum at 562 nm. It is commonly used as a reagent in the colorimetric BCA protein quantitation assay in which Cu2+ is converted to Cu+ as a function of protein concentration under alkaline conditions. | | Chemical Properties |
White to light yellow powder.
IUPAC Name is disodium [2,2'-biquinoline]-4,4'-dicarboxylate
| | Uses | For determination of Cu and protein assays.Bicinchoninic acid disodium salt is useful for the determination of copper. It is also used for the determination of protein concentration. | | Uses | - Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases.
- It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose).
- It has been used for determining protein concentration.
| | Application | 2,2'-Bicinchoninic Acid Disodium Salt is a photometric reagent for protein assay. It is used for determination of reducing sugar and protein via Cu(I). Also a specific reagent for Cu(I) with sensitivity similar to that of neocuproin. | | Biochem/physiol Actions | Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+), in presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration. | | References | [1] P.K. SMITH. Measurement of protein using bicinchoninic acid[J]. Analytical biochemistry, 1985, 150 1: Pages 76-85. DOI: 10.1016/0003-2697(85)90442-7
[2] BRENNER A.J. Harris E D. A Quantitative Test for Copper Using Bicinchoninic Acid[J]. Analytical biochemistry, 1995, 226 1: Pages 80-84. DOI: 10.1006/abio.1995.1194
[3] WALKER J M. The bicinchoninic acid (BCA) assay for protein quantitation.[J]. Methods in molecular biology, 1994, 32: 5-8. DOI: 10.1385/0-89603-268-x:5 |
| | Bicinchoninic Acid Disodium Salt Preparation Products And Raw materials |
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