| Company Name: |
Sigma-Aldrich
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021-61415566 800-8193336 |
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| Products Intro: |
Product Name:DAF-FM
CAS:254109-20-1
Purity:>=98% (HPLC) Package:1MG Remarks:D1821-1MG
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| Company Name: |
Alfa Chemistry
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| Tel: |
1-516-6625404 |
| Email: |
support@alfa-chemistry.com |
| Products Intro: |
Product Name:DAF-FM
CAS:254109-20-1
Purity:98%+ Package:1g;10g;100g;1KG;5KG
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| Product Name: | DAF-FM | | Synonyms: | DAF-FM;DIAMINOFLUORESCEIN-FM;4-AMINO-5-METHYLAMINO-2',7'-DIFLUOROFLUORESCEIN;3-AMINO, 4-AMINOMETHYL-2',7'-DIFLUOROFLUORESCEIN;4-Amino-5-methylamino-2′,7′-difluorescein;DAF-FM solution;4-amino-5-methylamino-20,70-difluorodifluorofluorescein;PPRXFLLOWKZHMK-UHFFFAOYSA-N | | CAS: | 254109-20-1 | | MF: | C21H14F2N2O5 | | MW: | 412.34 | | EINECS: | | | Product Categories: | Xanthene | | Mol File: | Mol File |  |
| | DAF-FM Chemical Properties |
| Melting point | 265 ℃ | | storage temp. | -20°C | | solubility | DMSO: >1mg/mL | | pka | 8.19 ± 0.20, most acidic, temperature:
25 °C; 4.23 ± 0.20, most basic(at 25℃) | | form | Solid | | color | clear colorless to yellow | | Appearance | yellow to brown solid | | λmax | 487 nm, 495 nm | | Biological Applications | Nitric oxide indicator; detecting microorganisms; measuring Ga i-coupled or Gao-coupled receptors activity | | InChI | 1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3 | | InChIKey | DIJCILWNOLHJCG-UHFFFAOYSA-N | | SMILES | Fc1cc2c(cc1O)Oc3c(cc(c(c3)O)F)C42OC(=O)c5c4ccc(c5N)NC |
| Hazard Codes | Xn | | Risk Statements | 22 | | WGK Germany | 3 | | Storage Class | 11 - Combustible Solids |
| | DAF-FM Usage And Synthesis |
| Description | DAF-FM (4-amino-5-methylamino-2′,7′-difluorofluorescein) is a cell-impermeant, fluorescent probe for detecting and quantifying low concentrations of nitric oxide (NO). DAF-FM does not need to be activated by cytosolic enzymes and is suitable to detect NO in extracellular matrix.
The fluorescence quantum yield of DAF-FM is ~0.005, but it increases about 160-fold to ~0.81 after reacting with NO and forming a fluorescent benzotriazole (excitation/emission maxima at 495/515 nm) .
The NO detection limit of DAF-FM (~3 nM) is more sensitive than that of DAF-2 (~5 nM). The fluorescence of the NO adduct of DAF-FM is independent of pH above pH 5.5. Moreover, the NO adduct of DAF-FM demonstrates a significantly enhanced photostability compared to that of DAF-2, ensuring reliable results and additional time for imaging.
DAF-FM should be dissolved in DMSO and then used to prepare a working solution. Buffers containing bovine serum albumin (BSA) or phenol red can affect the fluorescence and should be used cautiously.
The cell-permeant version of DAF FM — DAF-FM DA is also available. | | Uses | DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples. | | Uses | DAF-FM is important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. | | Uses | DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells. | | Biochem/physiol Actions | DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes. |
| | DAF-FM Preparation Products And Raw materials |
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