Alexa Fluor 647 NHS Ester manufacturers
- AF 647 NHS ester
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2025-06-07
- CAS:1620475-28-6
- Min. Order: 10mg
- Purity: >95.00%
- Supply Ability: 10mg
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| | Alexa Fluor 647 NHS Ester Basic information |
| Product Name: | Alexa Fluor 647 NHS Ester | | Synonyms: | Alexa Fluor? 647 NHS Ester;OKF647(C5) SE.TEA;Alexa fluor 647 NHS ester (TEA)NHS;AF 647 NHS ester;3-(2-(5-(3,3-Dimethyl-5-sulfo-1-(3-sulfopropyl)indolin-2-ylidene)penta-1,3-dien-1-yl)-3-(5-((2,5-dioxopyrrolidin-1-yl)oxy)-5-oxopentyl)-3-methyl-5-sulfo-3H-indol-1-ium-1-yl)propane-1-sulfonate | | CAS: | 1620475-28-6 | | MF: | C39H47N3O16S4 | | MW: | 942.05 | | EINECS: | | | Product Categories: | | | Mol File: | 1620475-28-6.mol |  |
| | Alexa Fluor 647 NHS Ester Chemical Properties |
| solubility | good in water, DMSO | | Appearance | dark violet solid | | InChIKey | WTYBXOVGGRVXPB-UHFFFAOYSA-N | | SMILES | C(C1(C(=[N+](CCCS([O-])(=O)=O)C2=CC=C(S(O)(=O)=O)C=C12)C=CC=CC=C1C(C2=CC(S(O)(=O)=O)=CC=C2N1CCCS(O)(=O)=O)(C)C)C)CCCC(=O)ON1C(CCC1=O)=O |
| | Alexa Fluor 647 NHS Ester Usage And Synthesis |
| Description | AF 647 NHS ester is a bright, far-red fluorescent dye, used for cellular visualization and labeling of antibodies, peptides, proteins, modified oligonucleotides, and other amine-containing molecules.
Due to the lack of significant self-quenching AF 647 dye molecules can be conjugated to proteins at high molar ratios, resulting in the sensitive detection of low-abundance biomolecules potential.
AF 647 NHS ester dye possesses high water-solubility and high fluorescence quantum yield; it is pH-insensitive over a wide molar range. Widely applied for imaging and flow cytometry. | | Uses | Alexa fluor 647 NHS ester can be used to label Alexa fluor 647 to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules[1]. | | References | [1] Martin Lehmann, et al. Multicolor Caged dSTORM Resolves the Ultrastructure of Synaptic Vesicles in the Brain. Angew Chem Int Ed Engl. 2015 Nov 2;54(45):13230-5. DOI:10.1002/anie.201505138 | | Abs/Em Maxima | 655/680 nm | | Fluoresene quantum yield | 0.15 | | Extinction Coefficient | 191800 | | CF260 | 0.09 | | CF280 | 0.08 |
| | Alexa Fluor 647 NHS Ester Preparation Products And Raw materials |
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