- Biotin NHS
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- $45.00 / 100mg
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2025-07-27
- CAS:35013-72-0
- Min. Order:
- Purity: 98.63%
- Supply Ability: 10g
- Biotin NHS
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- $45.00 / 100mg
-
2025-06-09
- CAS:35013-72-0
- Min. Order:
- Purity: 98.63%
- Supply Ability: 10g
- Biotin-NHS ester
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- $0.00 / 25g
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2025-06-07
- CAS:35013-72-0
- Min. Order: 25g
- Purity: >95.00%
- Supply Ability: 25g
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| | BIOTIN-NHS Basic information |
| Product Name: | BIOTIN-NHS | | Synonyms: | (+)-biotin-n-hydroxysuccimide ester;n-hydroxysuccimidobiotin;BIOTIN-OSU: N-HYDROXYSUCCINIMIDOBIOTIN;d-Biotin NHS ester;(2,5-dioxopyrrolidin-1-yl) 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoate;5-(2-OXO-HEXAHYDRO-THIENO[3,4-D]IMIDAZOL-6-YL)-PENTANOIC ACID 2,5-DIOXO-PYRROLIDIN-1-YL ESTER;D-(+)BIOTIN N-HYDROXYSUCCINIMIDE ESTER;D-BIOTIN-N-HYDROXYSUCCINIMIDE ESTER | | CAS: | 35013-72-0 | | MF: | C14H19N3O5S | | MW: | 341.38 | | EINECS: | 609-055-0 | | Product Categories: | biotinyl;BiotinylationReagents;Biotinylation Reagents;Cross Linking Reagents;Biotin Derivatives;PEG | | Mol File: | 35013-72-0.mol |  |
| | BIOTIN-NHS Chemical Properties |
| Melting point | 212-214 °C | | density | 1.45±0.1 g/cm3(Predicted) | | storage temp. | -20°C | | solubility | DMF: ≤50 mg/mL Stable for at least one month in dry DMF | | form | powder | | pka | 13.89±0.40(Predicted) | | color | White to off-white | | BRN | 4720781 | | Stability: | Stable. Incompatible with strong oxidizing agents. | | InChI | InChI=1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1 | | InChIKey | YMXHPSHLTSZXKH-RVBZMBCESA-N | | SMILES | C1(=O)N[C@]2([H])[C@H](CCCCC(ON3C(=O)CCC3=O)=O)SC[C@]2([H])N1 | | CAS DataBase Reference | 35013-72-0(CAS DataBase Reference) |
| | BIOTIN-NHS Usage And Synthesis |
| Description | N-Hydroxysuccinimide (NHS) activates carboxylic acid groups (on biotin) to facilitate coupling reactions. Biotin-NHS is a compound used to attach biotin to primary amines under alkaline conditions (pH~8-9). For example, lysines on the surface of proteins, including antibodies, are ideal targets for biotinylation with this compound. | | Chemical Properties | White Solid | | Uses | amine reactive biotinylation reagent | | Uses | Biotin-NHS is used in the biotinylation of proteins and peptides. Couples with primary amine in the pH range 6.5-8.5. | | Uses | Amino reactive biotin reagent for preparing biotinylated surfaces or polypeptides. | | reaction suitability | reaction type: Biotinylations | | Synthesis | GENERAL STEPS: To a mixed solution of D-biotin (0.82 mmol, 200 mg) and N-hydroxybutanediimide (0.89 mmol, 102 mg) was added 3-(ethyliminomethylamino)-N,N-dimethylpropan-1-amine hydrochloride (0.96 mmol, 184 mg). The reaction mixture was stirred at room temperature overnight and then concentrated to give a white solid. The crude product was mixed with isopropanol, heated to 70 °C and cooled. The pure product was obtained by filtration as a white powder in 91% yield (0.75 mmol, 255 mg). | | Purification Methods | Recrystallise the ester from refluxing isoPrOH and dry it in a vacuum over P2O5 + KOH. [Jasiewicz et al. Exp Cell Biol 100 213 1976.] | | References | [1] Journal of the American Chemical Society, 2007, vol. 129, # 45, p. 13987 - 13996
[2] New Journal of Chemistry, 2013, vol. 37, # 11, p. 3762 - 3769
[3] Organic Letters, 2013, vol. 15, # 16, p. 4130 - 4133
[4] Journal of Materials Chemistry, 2004, vol. 14, # 17, p. 2638 - 2642
[5] Beilstein Journal of Organic Chemistry, 2015, vol. 11, p. 784 - 791 |
| | BIOTIN-NHS Preparation Products And Raw materials |
| Raw materials | 1H-Imidazole, 1-[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]--->TSTU-->D-Biotin-->N-Hydroxysuccinimide | | Preparation Products | biotinylamidoethylacetamide-->LC-LC(+)-Biotin-->N-BIOTINYL-3,6,9-TRIOXAUNDECANE-1,11-DIAMINE-->D-BIOTINOL-->(+)-BIOTIN-EPSILON-AMINOCAPROIC ACID-->FMOC-LYS(BIOTIN)-OH-->4-((Biotinoyl)amino)Butyric acid-->N-[6-[[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1 -oxopentyl]amino]hexyl]-carbamic Acid 1,1-Dimethylethyl Ester |
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