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| | ROS Detection Cell-Based Assay Kit (DCFDA) Basic information |
| | ROS Detection Cell-Based Assay Kit (DCFDA) Chemical Properties |
| | ROS Detection Cell-Based Assay Kit (DCFDA) Usage And Synthesis |
| Description | 2,7-Dichlorofluoroscin Diacetate (DCFDA or 2,7-dichlorodihydrofluoroscein diacetate) is a widely-used, cell-permeable redox sensitive fluorescent probe that is oxidized by ROS and certain RNS to yield the highly fluorescent product 2,7-dichlorofluoroscein. Cayman’s ROS Detection Cell-Based Assay Kit (DCFDA) uses DCFDA as a fluorescent probe for the detection of ROS generation. Pyocyanin, a cell-permeable compound capable of redox cycling, is included as a positive control for ROS generation. N-acetyl-cysteine is included as an antioxidant control. | | References | [1] TING-YUN HUANG. Furanocoumarin Notopterol: Inhibition of Hepatocellular Carcinogenesis through Suppression of Cancer Stemness Signaling and Induction of Oxidative Stress-Associated Cell Death.[J]. Nutrients, 2023, 15 11. DOI: 10.3390/nu15112447
[2] PRATIBHA BOMBLE Bimalendu B N. Differential manifestation of RONS and antioxidant enzymes in response to singular versus combinatorial stress in Chironomus ramosus.[J]. Stress biology, 2022, 2 1: 56. DOI: 10.1007/s44154-022-00077-8
[3] ELIANA STEINBERG. Methionine aminopeptidase 2 as a potential target in pancreatic ductal adenocarcinoma.[J]. Accounts of Chemical Research, 2022: 6243-6255.
[4] EMMANUELA N. BONGLACK. Monocarboxylate transporter antagonism reveals metabolic vulnerabilities of viral-driven lymphomas[J]. Proceedings of the National Academy of Sciences, 2020, 39 1. DOI: 10.1101/2020.12.04.410563 |
| | ROS Detection Cell-Based Assay Kit (DCFDA) Preparation Products And Raw materials |
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