| Company Name: |
Enzo Biochem Inc |
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Enzo Biochem Inc. 13797054060 |
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Enzoname@qq.com |
| Company Name: |
huaxingbio |
| Tel: |
1-4006-909-904 13121892008 |
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huaxingbio@163.com |
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| | GFAP Polyclonal Antibody Basic information |
| | GFAP Polyclonal Antibody Chemical Properties |
| | GFAP Polyclonal Antibody Usage And Synthesis |
| Description | Glial fibrillary acidic protein (GFAP) is a protein encoded by the GFAP gene in humans and a member of the class III intermediate filament (IF) protein family. It is composed of an N-terminal head domain, a highly conserved α-helical rod domain, and a C-terminal tail domain that mediate GFAP self-assembly, dimerization, and oligomerization, respectively. GFAP is expressed in, and has commonly been used as a pan marker for, mature astrocytes. GFAP IFs form a dynamic network of cytosolic filament proteins that collectively provide structure and strength to the cytoskeleton of astrocytes, thus supporting their morphology and function. Isolated astrocytes from neonatal Gfap-/- mouse brain have reduced numbers of IFs and IF bundles, increased proliferation, and loss of contact-inhibited growth. Gfap-/- mice develop more diffuse and infiltrative brain lesions compared to wild-type littermates in a mouse model of experimental autoimmune encephalomyelitis (EAE). Mutations in the rod and tail domains of GFAP have been associated with Rosenthal fiber formation, a hallmark of Alexander disease. Transgenic overexpression of Gfap in mice increases the expression of certain cytokines and antioxidative enzymes in the olfactory bulb and has been used as a mouse model of Alexander disease. GFAP can be citrullinated on the arginine residue at position 270 (R270) and at R416 by protein arginine deiminase 1 (PAD1; ) and PAD2 . Citrullinated GFAP has been found in rat cerebral cortex in a model of traumatic brain injury, as well as in postmortem hippocampus from patients with Alzheimer''s disease. Cayman''s GFAP Polyclonal Antibody can be used for ELISA, IHC, and WB applications. The antibody recognizes GFAP at ~50 kDa from human and murine samples. | | References | [1] ELLY M HOL Yassemi C. Type III Intermediate Filaments Desmin, Glial Fibrillary Acidic Protein (GFAP), Vimentin, and Peripherin.[J]. Cold Spring Harbor perspectives in biology, 2017, 9 12. DOI: 10.1101/cshperspect.a021642
[2] MASAKI INAGAKI. Glial Fibrillary Acidic Protein: Dynamic Property and Regulation by Phosphorylation[J]. Brain Pathology, 1994, 4 3: 239-243. DOI: 10.1111/j.1750-3639.1994.tb00839.x
[3] W J CHEN R K L. The endless story of the glial fibrillary acidic protein.[J]. Journal of cell science, 1994, 107 ( Pt 8): 2299-2311. DOI: 10.1242/jcs.107.8.2299
[4] MILOS PEKNY . GFAP-Deficient Astrocytes Are Capable of Stellationin VitroWhen Cocultured with Neurons and Exhibit a Reduced Amount of Intermediate Filaments and an Increased Cell Saturation Density[J]. Experimental cell research, 1998, 239 2: Pages 332-343. DOI: 10.1006/excr.1997.3922
[5] J T RUTKA S L S. Transfection of human astrocytoma cells with glial fibrillary acidic protein complementary DNA: analysis of expression, proliferation, and tumorigenicity.[J]. Cancer research, 1993, 53 15: 3624-3631.
[6] W LIEDTKE. Experimental autoimmune encephalomyelitis in mice lacking glial fibrillary acidic protein is characterized by a more severe clinical course and an infiltrative central nervous system lesion.[J]. American Journal of Pathology, 1998, 152 1: 251-259.
[7] RONG LI . GFAP mutations in Alexander disease[J]. International Journal of Developmental Neuroscience, 2002, 20 3: Pages 259-268. DOI: 10.1016/s0736-5748(02)00019-9
[8] TRACY L HAGEMANN. Gene expression analysis in mice with elevated glial fibrillary acidic protein and Rosenthal fibers reveals a stress response followed by glial activation and neuronal dysfunction.[J]. Human molecular genetics, 2005, 14 16: 2443-2458. DOI: 10.1093/hmg/ddi248
[9] AKIHITO ISHIGAMI. Mass spectrometric identification of citrullination sites and immunohistochemical detection of citrullinated glial fibrillary acidic protein in Alzheimer’s disease brains[J]. Journal of Neuroscience Research, 2015, 93 11: 1664-1674. DOI: 10.1002/jnr.23620
[10] RACHEL C LAZARUS. Protein Citrullination: A Proposed Mechanism for Pathology in Traumatic Brain Injury.[J]. Frontiers in Neurology, 2015: 204. DOI: 10.3389/fneur.2015.00204 |
| | GFAP Polyclonal Antibody Preparation Products And Raw materials |
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