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| | PathScan Total IκBα Sandwich ELISA Kit Chemical Properties |
| | PathScan Total IκBα Sandwich ELISA Kit Usage And Synthesis |
| Description | PathScan® Total IκBα Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total IκBα protein. An IκBα Mouse mAb has been coated onto the microwells. After incubation with cell lysates, both nonphospho- and phospho-IκBα proteins are captured by the coated antibody. Following extensive washing, an IκBα Rabbit Antibody is added to detect the captured IκBα protein. Anti-rabbit IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of total IκBα protein. | | Reactivity | Human;Mouse | | Background | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified. | | References | [1] Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
[2] Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
[3] Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
[4] Brown, K. et al. (1995) Science 267, 1485-8.
[5] Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
[6] Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
[7] Chen, Z.J. et al. (1996) Cell 84, 853-62.
[8] Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.
[9] Chen, J. et al. (2008) J Cell Mol Med , . |
| | PathScan Total IκBα Sandwich ELISA Kit Preparation Products And Raw materials |
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