| Company Name: |
Shanghai Universal Biotech Co.,Ltd
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| Tel: |
15921930842 15921930842 |
| Email: |
yh-wang@univ-bio.com |
| Products Intro: |
Product Name:BRD4 (E2A7X) Rabbit mAb
Purity:见优宁维官网 Package:100ul/RMB 1
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| Company Name: |
Cell Signaling Technology Inc
|
| Tel: |
21-80243558 86218024 |
| Email: |
info@cellsignal.cn |
| Products Intro: |
Product Name:BRD4 (E2A7X) Rabbit mAb
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| | BRD4 (E2A7X) Rabbit mAb Chemical Properties |
| | BRD4 (E2A7X) Rabbit mAb Usage And Synthesis |
| Source | Rabbit | | Reactivity | Human | | Background | Bromodomain-containing protein 4 is a member of the bromodomains and extra terminal family of proteins, which also includes BRD2, BRD3, and BRDT. BET family proteins contain two tandem bromodomains and an extra terminal domain, and bind acetyl lysine residues. BRD4 is a chromatin-binding protein with a preference for Lys14 on histone H3 as well as Lys5 and Lys12 on histone H4. BRD4 chromatin binding occurs throughout the cell cycle, including condensed mitotic chromosomes, when the majority of genes are silenced. BRD4 association with chromatin during mitosis is thought to be an important part of the bookmarking mechanism to accelerate reactivation of the silenced genes upon exit from mitosis. BRD4 has been shown to facilitate transcription by recruiting the positive transcription elongation factor b complex that phosphorylates Ser2 of the heptapeptide repeat of the carboxy-terminal domain of RNA polymerase II, promoting transcription elongation. In addition, BRD4 has been found to be part of the super elongation complex and the polymerase associated factor complex in MLL-fusion derived leukemia cell lines, demonstrating a role for BRD4 in the regulation of transcription elongation. Research studies have shown that BRD4 may be promising therapeutic targets for various Myc-driven cancers, such as Burkitt’s lymphoma and certain acute myeloid leukemias. Investigators have found molecular inhibition of BET proteins to be effective in inducing apoptosis in various MLL-fusion driven leukemic cell lines by competing BRD3 and BRD4 from chromatin, leading to reduced expression of Bcl-2, Myc, and CDK6. BET inhibition has also been shown to have antitumor activities against nuclear protein in testis midline carcinoma cell lines and xenografts in mice where BRD4 is found to be a frequent translocation partner of the NUT protein. In addition, BRD4 regulates the expression of some inflammatory genes, and inhibition of BRD4 chromatin binding causes reduced expression of a subset of inflammatory genes in macrophages, leading to protection against endotoxic shock and sepsis. | | References | [1] Belkina, A.C. and Denis, G.V. (2012) Nat Rev Cancer 12, 465-77.
[2] Voigt, P. and Reinberg, D. (2011) Genome Biol 12, 133.
[3] Wu, S.Y. and Chiang, C.M. (2007) J Biol Chem 282, 13141-5.
[4] Dey, A. et al. (2003) Proc Natl Acad Sci U S A 100, 8758-63.
[5] Dey, A. et al. (2009) Mol Biol Cell 20, 4899-909.
[6] Zhao, R. et al. (2011) Nat Cell Biol 13, 1295-304.
[7] Jang, M.K. et al. (2005) Mol Cell 19, 523-34.
[8] Yang, Z. et al. (2005) Mol Cell 19, 535-45.
[9] Dawson, M.A. et al. (2011) Nature 478, 529-33.
[10] Muller, S. et al. (2011) Expert Rev Mol Med 13, e29.
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| | BRD4 (E2A7X) Rabbit mAb Preparation Products And Raw materials |
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