MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH

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MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH Basic information
Product Name:MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH
Synonyms:MCA-(ALA7,LYS(DNP)9)-BRADYKININ;L-Lysine, N2-[2-(7-methoxy-2-oxo-2H-1-benzopyran-4-yl)acetyl]-L-arginyl-L-prolyl-L-prolylglycyl-L-phenylalanyl-L-seryl-L-alanyl-L-phenylalanyl-N6-(2,4-dinitrophenyl)-;N2-[2-(7-Methoxy-2-oxo-2H-1-benzopyran-4-yl)acetyl]-L-arginyl-L-prolyl-L-prolylglycyl-L-phenylalanyl-L-seryl-L-alanyl-L-phenylalanyl-N6-(2,4-dinitrophenyl)-L-lysine;(7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys-(2,4-dinitrophenyl);7-Methoxycoumarin-4-acetyl [Ala7-(2,4-Dinitrophenyl)Lys9]-Bradykinin;Matrix metalloproteinases MMP FRET Substrate V, bradykinin Mca- (Ala?, Lys(Dnp)?;Mca-(Ala7,Lys(Dnp)9)-Bradykinin trifluoroacetate salt;MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH
CAS:323577-36-2
MF:C66H81N15O19
MW:1388.44
EINECS:
Product Categories:
Mol File:323577-36-2.mol
MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH Structure
MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH Chemical Properties
density 1.48±0.1 g/cm3(Predicted)
storage temp. -15°C
pka3.38±0.10(Predicted)
form Solid
color Light yellow to yellow
AppearanceWhite or Off-white Lyophilized Powder
Safety Information
MSDS Information
MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH Usage And Synthesis
Description

Mca-(Ala7,Lys(Dnp)9)-Bradykinin is a very sensitive internally quenched fluorescent substrate for endothelin-converting enzyme-1 (ECE-1), a membrane-bound zinc metallopeptidase related to neprilysin in amino acid sequence. It is used as a substrate for insulin-degrading enzymes (IDE) and ACE2.

UsesMca-(ala7,lys(dnp)9)-bradykinin is a sensitive fluorogenic substrate for ECE-1 (endothelin-converting enzyme-1). The incorporation of a (7-methoxycoumarin-4-yl)acetyl (Mca) fluorescent group and a 2,4-dinitrophenyl (Dnp) quenching group has resulted in a large fluorescence increase upon substrate cleavage[1].
References[1] Johnson GD, et al. Development of an internally quenched fluorescent substrate selective for endothelin-converting enzyme-1. Anal Biochem. 2000 Nov 1;286(1):112-8. DOI:10.1006/abio.2000.4772
SequeneMca-RPPGFSAFK(Dnp)
MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(Dnp)-OH Preparation Products And Raw materials
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