|
|
| | MCL-1 ANTIBODY Chemical Properties |
| | MCL-1 ANTIBODY Usage And Synthesis |
| Source | Rabbit | | Reactivity | Human | | Background | Mcl-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway. Similar to other Bcl-2 family members, Mcl-1 localizes to the mitochondria, interacts with and antagonizes pro-apoptotic Bcl-2 family members, and inhibits apoptosis induced by a number of cytotoxic stimuli. Mcl-1 differs from its other family members in its regulation at both the transcriptional and posttranslational level. First, Mcl-1 has an extended amino-terminal PEST region, which is responsible for its relatively short half-life. Second, unlike other family members, Mcl-1 is rapidly transcribed via a PI3K/Akt dependent pathway, resulting in its increased expression during myeloid differentiation and cytokine stimulation. Mcl-1 is phosphorylated in response to treatment with phorbol ester, microtubule-damaging agents, oxidative stress, and cytokine withdrawal. Phosphorylation at Thr163, the conserved MAP kinase/ERK site located within the PEST region, slows Mcl-1 protein turnover but may prime the GSK-3 mediated phosphorylation at Ser159 that leads to Mcl-1 destabilization. Mcl-1 deficiency in mice results in peri-implantation lethality. In addition, conditional disruption of the corresponding mcl-1 gene shows that Mcl-1 plays an important role in early lymphoid development and in the maintenance of mature lymphocytes. | | References | [1] Kozopas, K.M. et al. (1993) Proc Natl Acad Sci USA 90, 3516-20.
[2] Yang, T. et al. (1995) J Cell Biol 128, 1173-84.
[3] Sato, T. et al. (1994) Proc Natl Acad Sci USA 91, 9238-42.
[4] Zhou, P. et al. (1997) Blood 89, 630-43.
[5] Wang, J.M. et al. (1999) Mol Cell Biol 19, 6195-206.
[6] Jourdan, M. et al. (2003) Oncogene 22, 2950-9.
[7] Chao, J.R. et al. (1998) Mol Cell Biol 18, 4883-98.
[8] Domina, A.M. et al. (2000) J Biol Chem 275, 21688-94.
[9] Inoshita, S. et al. (2002) J Biol Chem 277, 43730-4.
[10] Domina, A.M. et al. (2004) Oncogene 23, 5301-15.
|
| | MCL-1 ANTIBODY Preparation Products And Raw materials |
|