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| | c-Kit (Ab81) Mouse mAb Chemical Properties |
| | c-Kit (Ab81) Mouse mAb Usage And Synthesis |
| Source | Mouse | | Reactivity | Human | | Background | c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors. It plays a critical role in activation and growth in a number of cell types, including hematopoietic stem cells, mast cells, melanocytes, and germ cells. Upon binding with its stem cell factor ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components, including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders, and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors. Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo. | | References | [1] Martin, F.H. et al. (1990) Cell 63, 203-11.
[2] Yarden, Y. et al. (1987) EMBO J 6, 3341-51.
[3] Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
[4] Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.
[5] Nocka, K. et al. (1990) EMBO J 9, 1805-13.
[6] Hirota, S. et al. (1998) Science 279, 577-80.
[7] Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62. |
| | c-Kit (Ab81) Mouse mAb Preparation Products And Raw materials |
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