Pentafluorobenzenesulfonyl fluorescein NEW
| Price | $142 | $663 | $1130 |
| Package | 1mg | 5mg | 10mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2025-08-29 |
Product Details
| Product Name: Pentafluorobenzenesulfonyl fluorescein | CAS No.: 728912-45-6 |
| Supply Ability: 10g | Release date: 2025/08/29 |
Product Introduction
Bioactivity
| Name | Pentafluorobenzenesulfonyl fluorescein |
| Description | Pentafluorobenzenesulfonyl fluorescein itself no fluorescence, but were in the H2O2 in sulfonyl key fluoresce when hydrolyzed. Pentafluorobenzenesulfonyl fluorescein is often treated as selective H2O2 sensors, can be used for the detection of H2O2 content in the cell. |
| Cell Research | Instructions 1. Prepare probe solution: PFSF is usually dissolved in anhydrous DMSO to prepare a stock solution (e.g. 1–10 mM), and then diluted to a working concentration (usually 1–10 µM) according to experimental requirements. 2. Sample treatment: 1) For cell experiments, PFSF is added to the culture medium (usually used in phenol red-free culture medium containing 1% FBS to avoid fluorescence background interference), and then incubate the cells for 30 minutes to 1 hour at 37°C, away from light. 2) For chemical or solution experiments, PFSF can be directly mixed with the target system (such as a buffer solution containing H₂O₂). 3. Fluorescence detection: Fluorescence excitation wavelength: 490–500 nm, emission wavelength: 515–530 nm. The signal can be recorded using a fluorescence microscope, fluorescence spectrophotometer, or fluorescence plate reader. 4. Calibration and control: 1) It is recommended to set up a no-H₂O₂ control group and a H₂O₂ standard curve to verify signal specificity. 2) To ensure probe specificity, catalase can be added to eliminate H₂O₂ interference. Notes 1) Storage conditions: PFSF should be stored at -20°C in the dark and fully dissolved before use. 2) Experimental environment: Avoid high temperature and direct exposure to strong light to prevent probe decomposition or increased autofluorescence. 3) Selectivity verification: In addition to H₂O₂, the probe may have a slight reaction to other strong oxidants (such as peroxynitrite), and the specificity should be verified according to experimental requirements. |
| Storage | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO:PBS (pH 7.2)(1:1) : 0.25 mg/mL (0.44 mM), Sonication is recommended. DMSO : 30 mg/mL (53.34 mM), Sonication is recommended. Ethanol : 20 mg/mL (35.56 mM), Sonication is recommended. DMF : 30 mg/mL (53.34 mM), Sonication is recommended. |
| Keywords | Pentafluorobenzenesulfonyl fluorescein |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
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United States- Since: 2011-01-07
- Address: 36 Washington Street, Wellesley Hill, MA
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