脂肪酸结合蛋白5(FABP5)重组蛋白
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Fatty Acid Binding Protein 5, Epidermal
Gene Names: FABP5
Size: 100µg
Source: Recombinant
Expression Expression Expression Expression Host: E.coli
Function: Function: Function: Function: High specificity for fatty acids. Highest affinity for C18 chain length.
Decreasing the chain length or introducing double bonds reduces the affinity. May
be involved in keratinocyte differentiation.
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm.
Tissue Specificity: Specificity: Specificity: Specificity: Keratinocytes; highly expressed in psoriatic skin.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Ala2~Glu135 (Accession # Q01469), with a N-terminal His-tag.
Grade & Purity: Purity: Purity: Purity: >97%, 16.3 kDa as determined by SDS-PAGE reducing
conditions.
Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 16.3 kDa
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in PBS.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term
storage. Avoid repeated freeze/thaw cycles.
Valid period: period: period: period: 12 months stored at -80oC.
[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]
The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will
increase the chelating ability of the tag to Ni-Sepharose during purification.
MGHHHHHHSGS-ATVQQLEGR WRLVDSKGFD EYMKELGVGI ALRKMGAMAK PDCIITCDGK NLTIKTESTL
KTTQFSCTLG EKFEETTADG RKTQTVCNFT DGALVQHQEW DGKESTITRK LKDGKLVVEC VMNNVTCTRI
YEKVE
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Madsen P.S., et al. (1992) J. Invest. Dermatol. 99:299-305.
2. Gutierrez-Gonzalez L.H., et al. (2002) Biochem. J. 364:725-737.
3. Hohoff C., et al. (1999) Biochemistry 38:12229-12239.
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关键字： E-FABP;EFABP;PA-FABP;脂肪酸结合蛋白5(FABP5)重组蛋白;
上海博尔森生物科技有限公司成立于2020年09月02日，注册地位于上海市松江区石湖荡镇石湖新路95号。
上海博尔森生物科技有限公司虽然年轻，但朝气蓬勃，志向远大； 更是一家专注于生命科学与生物技术领域，集产品研发、生产、销售为一体的高科技公司，并立志以竞争力的价格向客户输送高质量的生物试剂。主要研究领域是生命科学，包括分子生物学、细胞生物学、免疫学试剂、重组蛋白，抗体，实验技术服务等多个应用领域。主要产品有：Elisa检测试剂盒、PCR检测试剂盒、抗体、重组蛋白，蛋白定制，细胞系，原代细胞，常用实验试剂，染色液等科研产品。