氯离子通道辅助蛋白1(CLCA1)重组蛋白
[ PROPERTIES ]
Residues: Asn416~Arg670
Tags: N-terminal His-Tag
Accession: A8K7I4
Host: E. coli
Subcellular Location: Secreted, extracellular space.
Cell membrane; Peripheral membrane protein;
Extracellular side.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 7.1
Predicted Molecular Mass: 28.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8
oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
NTISG CFNEVKQSGA IIHTVALGPS AAQELEELSK MTGGLQTYAS DQVQNNGLID
AFGALSSGNG AVSQRSIQLE SKGLTLQNSQ WMNGTVIVDS TVGKDTLFLI TWTMQPPQIL
LWDPSGQKQG GFVVDKNTKM AYLQIPGIAK VGTWKYSLQA SSQTLTLTVT SRASNATLPP
ITVTSKTNKD TSKFPSPLVV YANIRQGASP ILRASVTALI ESVNGKTVTL ELLDNGAGAD
ATKDDGVYSR YFTTYDTNGR
[ REFERENCES ]
1. Bailey S.D., et al. (2010) Diabetes Care 33:2250-2253.
2. Hauber H.P., et al. (2010) J. Cyst. Fibros. 9:277-279.
3. Wu W., et al. (2010) Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 24:193-5,
2010:199-199.
4. Han S., et al. (2010) Hum. Immunol. 71:727-730.
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关键字： CaCC1;CLCRG1;Chloride;氯离子通道辅助蛋白1(CLCA1)重组蛋;
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BIOESN研究领域及主要产品 :
主要研究领域是生命科学，包括分子生物学、细胞生物学、免疫学试剂、重组蛋白，抗体，实验技术服务等多个应用领域。主要产品有：Elisa检测试剂盒、PCR检测试剂盒、抗体、重组蛋白，蛋白定制，细胞系，原代细胞，常用实验试剂，染色液等科研产品。