产品名称：磷酸二酯酶I 来源于东部菱背响尾蛇毒液

规格或纯度：EnzymoPure™, ≥20 units/mg dry weight

产品介绍：

Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg2+.Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP.

级别：EnzymoPure™

浓度：≥20 units/mg dry weight

储存温度：-20°C储存

运输条件：超低温运输

CAS编号和信息：9025-82-5

酶学委员会编号：3.1.4.1

单位定义：Unit Definition: One Unit hydrolyzes one micromole of p-nitrophenyl thymidine-5-phosphate per minute at 25°C, pH 8.9.

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