基本描述
产品名称：核糖核酸酶T1 来源于米曲霉(色谱纯化,冻干)
规格或纯度：EnzymoPure™, ≥2,500 units/mg
产品介绍：
Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3'-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA.Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .
Accession #：P00651
级别：EnzymoPure™
产品规格参数
浓度：≥2,500 units/mg
储存温度：2-8°C储存
运输条件：低温运输
CAS编号和信息：9026-12-4
酶学委员会编号：3.1.27.3
单位定义：One Unit releases the equivalent of one A260 unit of acid-soluble products from yeast RNA in 15 minutes at 37°C, pH 7.5
功能和特点
Accession #：P00651
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/R128612.html
关键字： RNase T1;Guanyloribonuclease;Ribonucleate 3′-guanylo-oligonucleotidohydrolase;RNase T1;Guanyloribonuclease;Ribonucleate 3′-guanylo-oligonucleotidohydrolase
上海阿拉丁生化科技股份有限公司是A股上市公司（（股票代码：688179），专注于科研试剂的研发、生产和销售，已陆续建立多个工厂和研发中心。作为领军企业，阿拉丁始终坚持质量第一，连续13年被评为“最受欢迎试剂品牌”。
阿拉丁目前常备库存试剂产品品种超过7万种，SKU总数超过46万，产品线涵盖了化学试剂、生化试剂、药靶配体、蛋白质和抗体等多个领域，是国内少数化学试剂到生物试剂全面发展的国产试剂品牌，产品同步发布在我们国内外电商平台。