Description
This assay employs the quantitative sandwich enzyme immunoassay technique. A Romiplostim protein has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Anti- Romiplostim antiobody present is bound by the immobilized Romiplostim protein. After washing away any unbound substances, a biotin-labeled Romiplostim protein is added to the wells. Following a wash to remove any unbound protein-biotin reagent, a streptavidin- horseradish peroxidase is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of antibody that anti-Romiplostim protein bound in the initial step.
Catalog No.
KAD76501
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 10%. For unopened kits, all reagents are stored as shown on the label of the reagent bottle, please store the Standard, Detection A, Detection B, and Capture Plate at - 20 ℃, the rest reagents should be store at 4℃.
Detection method
Colorimetric
Precision
Intra-Assay Precision (Precision within an assay): <12%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision.
Sample type
Plasma, Serum
Assay type
Quantitative
Sensitivity
0.025 μg/mL
Range
0.03125 μg/mL - 2 μg/mL
Recovery
80-120%
Shipping
2-8 ℃
Note
For Research Use Only.
关键字： Romiplostim ;Nplate;ELISA ;ELISA Kit;
武汉佰乐博生物（Biolab Reagents）始创于 2021 年，核心创始团队由五位毕业于武汉大学、华中科技大学、华中农业大学等知名高校的生命科学领域顶尖研究者组成。团队成员均深耕行业二十载，积淀了深厚的产品开发实力与成熟的技术应用经验，为企业发展筑牢核心技术根基。
佰乐博生物是国家高新技术企业、国家科技型中小企业，技术实力获权威认可；同时通过 ISO9001 质量管理体系、ISO13485 医疗器械质量管理体系双认证，以标准化、规范化的品控体系，筑牢产品品质基石。公司依托全球领先的技术平台，专注于高品质生物试剂的全球化开发与整合，与法国 AntibodySystem 品牌达成深度战略合作，系其在亚洲的独家授权合作伙伴以及生工生物KleanAB品牌的代理商。目前已构建起近 60000 种生命科学试剂的丰富产品矩阵，核心产品涵盖蛋白、抗体、试剂盒三大品类，重点覆盖肿瘤、神经系统等疾病研究热门领域，可全维度满足各类科研实验的多元需求。
自成立以来，佰乐博生物始终秉持初心，致力于为广大生命科学科研工作者、生物医药工业客户提供专业、全面、可靠的试剂产品与技术支持，以优质科研工具赋能科研探索，助力生命科学研究领域实现创新发展与深度突破。