基本描述
产品名称:Recombinant Mouse TNF-alpha Protein
规格或纯度:ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE&HPLC)
产品介绍:
肿瘤坏死因子α (TNF-α),又称Cachectin,是肿瘤坏死因子家族中最知名的成员,可导致细胞死亡。该蛋白由中性粒细胞、活化淋巴细胞、巨噬细胞、NK细胞、LAK细胞、星形胶质细胞、内皮细胞、平滑肌细胞和一些转化细胞产生。TNF-α的自然形式是糖基化的,但非糖基化的重组TNF-α具有类似的生物活性。人和鼠TNF-α在氨基酸水平上显示了约79%的同源性,并在两种物种之间显示了交叉反应性。已经描述了TNF-α的两种受体类型,几乎所有研究的细胞类型都显示存在一种或两种受体类型。重组小鼠TNF-α是一条含有157个氨基酸的非糖基化多肽链 。
生物活性:Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (Catalog # A113142). The ED₅₀ for this effect is typically <0.1 ng/mL.
纯度:>97% (SDS-PAGE; HPLC )
氨基酸:80-235aa
蛋白长度:Protein fragment
无载体:Yes
无动物源:Yes
Accession #:P06804
预测分子量:17.4 kDa
级别:无动物源, 无载体, 生物活性, ActiBioPure™, 无叠氮钠, 高性能
产品规格参数
储存缓冲液:Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4
复溶:1.This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. 2. Reconstitute in a sterile aqueous buffer to an appropriate concentration。 Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
储存温度:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:交付后等分,储存于 -20°C,避免冷冻/解冻循环。冻干,无添加剂。本品为活性蛋白质,可能会引起体内生物反应,请谨慎处理。
功能和特点
Accession #:P06804
生物活性:Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (Catalog # A113142). The ED₅₀ for this effect is typically <0.1 ng/mL.
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Protein Bioactivity: Recombinant Mouse TNF-alpha Protein (rp154759)
Recombinant Mouse TNF-alpha Protein (rp154759)-Protein Bioactivity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (Catalog # A113142). The EC₅₀ for this effect is typically <0.1ng/mL.

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SDS-PAGE: Recombinant Mouse TNF-alpha Protein (rp154759)
Recombinant Mouse TNF-alpha Protein (rp154759)-SDS-PAGE
3μg/lane of Recombinant Mouse TNF-alpha was resolved with SDS-PAGE under reducing (R) conditions and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 16.3 kDa.
查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/rp154759.html



关键字: 重组小鼠 TNF-alpha 蛋白;Recombinant Murine Tumor Necrosis Factor alpha (rMuTNF-α) ; APC1 protein ; Cachectin
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