基本描述
产品名称：Streptavidin protein (FITC)
规格或纯度：无叠氮钠, Ex:498nm, Em:517nm, Streptavidin Concentration: 1 mg/mL
应用：IF/ICC, Flow Cytometry
产品介绍：
背景
链霉亲和素（Streptavidin）是由streptomyces avidinii菌分泌的四聚体蛋白，可以高度特异性地和生物素（Biotin）结合，结合常数为10⁻¹⁴ mol/L，一分子 Streptavidin 可以结合 4 个 Biotin 分子，这种结合是目前自然界已知最强的非共价作用。 Streptavidin 和鸡蛋清来源的亲和素（Avidin）相比，两者与 Biotin 结合的亲和力高度相似性， 但Streptavidin在中性条件下几乎不带电荷，因此Streptavidin的非特异性结合比Avidin低很多， 检测时的非特异性背景也就少很多。Streptavidin 可以用于 Biotin 标记的抗体、核酸、蛋白或其他生物素标记分子的检测。Streptavidin (FITC)产品是用高纯度的 Streptavidin 和高纯度的 FITC 染料共价连接，并去除游离FITC。FITC标记的链霉亲和素是间接法荧光染色中一种非常有用的二抗，可以高亲和性地结合生物素化抗体。FITC由488nm的激光激发，可以在最大波长为520nm处检测到其发射光。
推荐工作浓度
Flow cytometry: 1:500-1:5000
IF:
1:50-1:1000
蛋白长度：Full length protein
偶联：FITC
级别：无叠氮钠, Ex:498nm, Em:517nm
产品规格参数
应用： Flow Cytometry; IF;
储存缓冲液：PBS (pH 7.4) buffer containing 1% BSA and 0.05% ProClin™ 300 as a preservative.
浓度：Streptavidin Concentration: 1 mg/mL
储存温度：2-8°C储存,避光,禁止冷冻
运输条件：低温运输,禁止冷冻
稳定性与储存：Shipped at 4℃. Store at 4°C and protected from prolonged exposure to light. Do not freeze.
功能和特点
图片
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ICC/IF-Streptavidin protein (FITC) (rp155876)
ICC/IF-Streptavidin protein (FITC) (rp155876)
ICC/IF with Streptavidin protein (FITC) (rp155876) stained HeLa cells. The cells were methanol fixed (5 min) and blocked with 1% BSA . The cells were then incubated with Biotin-γ-actin antibody overnight at 4°C. Then Streptavidin protein (FITC) rp155876 (green) was used at 1:1000 for 1h. DAPI was used to stain the cell nuclei (blue) .
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ICC/IF-Streptavidin protein (FITC) (rp155876)
ICC/IF-Streptavidin protein (FITC) (rp155876)
ICC/IF with Streptavidin protein (FITC) (rp155876) stained HeLa cells. The cells were methanol fixed (5 min) and blocked with 1% BSA . The cells were then incubated with Biotin-γ-actin antibody overnight at 4°C. Then Streptavidin protein (FITC) rp155876 (green) was used at 1:1000 for 1h. DAPI was used to stain the cell nuclei (blue) .
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Flow-Streptavidin protein (FITC) (rp155876)
Flow-Streptavidin protein (FITC) (rp155876)
BALB/c splenocytes stained with Biotin-CD45R antibody, followed by Streptavidin protein (FITC) (rp155876) at 1/2500 dilution.
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IF: Streptavidin Protein (FITC) (rp155876)
Streptavidin Protein (FITC) (rp155876) - IF
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Streptavidin Protein (FITC) (rp155876) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/rp155876.html
关键字： 链霉亲和素蛋白 (FITC);FITC Streptavidin ; Streptavidin-FITC ; FITC-streptavidin conjugate
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