基本信息
产品名称：Vinculin Mouse mAb
规格或纯度：无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
应用：WB, IF/ICC, Flow Cytometry
特异性：Vinculin
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原：Recombinant full length protein corresponding to Human Vinculin
阳性对照：WB: HeLa, HEK293, HepG2, A431, MCF-7, HUVEC, SK-N-SH, NIH/3T3, C6 whole cell lysates, Mouse lung lysates Flow: HeLa cells ICC/IF: HeLa cells
偶联：Unconjugated
产品属性
克隆类型：单克隆抗体
克隆号：AB01/1F7
Format：Whole IgG
亚型：IgG1
轻链亚型：kappa
SDS-PAGE：150 kDa
纯化方法：Protein A purified
物理外观：Liquid
储存缓冲液：10mM PBS, pH 7.2
防腐剂：No
浓度：见COA
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：在 +4°C 下短期储存（1-2 周）。-20°C或-80°C储存。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属：小鼠(Mouse)
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
克隆号：AB01/1F7
偶联：Unconjugated
图片
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Western Blot: Vinculin Mouse mAb (Ab169192)
Vinculin Mouse mAb (Ab169192) - Western Blot
All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 124.0 kDa
Observed band size: 125.8 kDa
Exposure time: 6.879 s
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Western Blot: Vinculin Mouse mAb (Ab169192)
Vinculin Mouse mAb (Ab169192) - Western Blot
All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL
Samples: Lysate at 20 µg
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 124.0 kDa
Observed band size: 122.0 kDa
Exposure time: 40.1 s
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Western Blot: Vinculin Mouse mAb (Ab169192)
Vinculin Mouse mAb (Ab169192) - Western Blot
All lanes: Vinculin Antibody (Ab169192) at 0.5 μg/mL
Samples: Lysate at 20 µg
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 124.0 kDa
Observed band size: 122.5 kDa
Exposure time: 0.1 s
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Flow Cytometry: Vinculin Mouse mAb (Ab169192)
Vinculin Mouse mAb (Ab169192) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vinculin Mouse mAb (Ab169192) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
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ICC/IF: Vinculin Mouse mAb (Ab169192)
Vinculin Mouse mAb (Ab169192) - ICC/IF
IF analysis of Vinculin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Vinculin Mouse mAb (Ab169192) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab169192.html
关键字： 黏着斑蛋白抗体 ; 黏着斑蛋白 ; Anti-vinculin 抗体 ; 粘着斑蛋白小鼠mAb
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