基本信息
产品名称:Rabbit Anti-Mouse IgG antibody
规格或纯度:见COA
应用:WB, EIA, IHC, CLIA, LF
应用:WB,CLIA,IHC,LF,EIA
种属反应性:小鼠(Mouse)
偶联:Unconjugated
产品属性
克隆类型:多克隆抗体
Format:Whole IgG
纯化方法:antigen affinity chromatography
物理外观:liquid
储存缓冲液:10mM phosphate buffered saline (PBS), pH7.4
浓度:见COA
储存温度:-20°C储存,避免反复冻融
运输条件:超低温运输
功能和特点
宿主种属:兔(Rabbit)
种属反应性:小鼠(Mouse)
偶联:Unconjugated
图片

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ELISA: Rabbit anti-mouse IgG antibody (Ab170451)
Rabbit anti-mouse IgG antibody (Ab170451) - ELISA
Rabbit anti-mouse IgG antibody (Ab170451) was tested by ELISA. The plate was coated with different amounts of mouse IgG (Ab170221) (3-fold started from 100 ng/mL). Rabbit anti-mouse IgG antibody was then added at a series of different dilution ratios. Anti-Rabbit IgG H&L (Biotin) (Ab136895) was used at 1/10000 dilution as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/10000 dilution for this ELISA.

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ELISA: Rabbit anti-mouse IgG antibody (Ab170451)
Rabbit anti-mouse IgG antibody (Ab170451) - ELISA
Cross-reactivity of Rabbit anti-mouse IgG antibody (Ab170451) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Rabbit anti-mouse IgG antibody was then added at different dilution ratio followed by incubation for 1h. Donkey Anti-Rabbit IgG H&L (Biotin) (Ab136895) was used at 1/10000 dilution as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/10000 dilution for this ELISA.
For the batch tested, Rabbit anti-mouse IgG antibody (Ab170451) showed a cross-reactivity with Human IgG when the dilution ratio of Rabbit anti-mouse IgG antibody is less than 1/64000.
查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/Ab170451.html



关键字: 兔抗鼠 IgG 抗体
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