SpeB产品信息
别称：SpeB,Streptopain,Exotoxin type B,Streptococcal cysteine proteinase
物种：Streptococcus pyogenes
属性：Protein
标记：Unconjugated
产品描述（Product Details）
SpeB is a unique recombinantly produced cysteine protease that digests antibodies from many species and subclasses in the hinge region, producing a homogenous pool of Fab fragments. The enzyme requires reducing conditions for activity on IgG.Human, mouse, rat, goat and sheep IgG are digested by SpeB, yielding Fab and Fc fragments. The primary digestion site on human IgG1 is KTHT / CPPCPAPE.
应用说明（Application）
Purified and homogenous Fab fragments can be used in many applications, including:
1. Study monovalent binding of Fab fragments to the antigen.
2. Elimination of Fc-mediated effector functions.
3. Reduced unspecific binding from Fc interactions.
单位定义（Unit Definition）
One unit digests ≥ 95% of 1 µg human IgG1 when incubated in PBS with 5 mM DTT or TCEP, pH 7.4 at 37°C for 1 hour.
质量控制（Quility Control）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, 1 mM TCEP, pH7.0 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
This product is stable after storage at:
The product MUST be stored at -70°C or lower upon receipt.
-70°C for 12 months under sterile conditions.
SpeB分子背景
SpeB is derived from Streptococcus pyogenes. It is an important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. It also cleaves human IL1B precursor to form biologically active IL1B and can induce apoptosis in human monocytes and epithelial cells in vitro, reduces phagocytic activity in monocytic cells. Thus, it may play a role in bacterial colonization, invasion, and inhibition of wound healing.
关键字： SpeB;SpeB蛋白;SpeB重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group（股票代码：301080）是成立于2010年的跨国生物科技公司，是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球，横跨亚洲、北美洲、欧洲，在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家，与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。