基本信息
产品名称:S100B Mouse mAb
规格或纯度:无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
应用:ELISA, CLIA, FIA, LF, GICA, Detection Antibody, Capture Antibody
特异性:S100B
种属反应性:人(Human)
免疫原:Recombinant Human S100B Protein, C-His tag
偶联:Unconjugated
产品属性
克隆类型:单克隆抗体
克隆号:22G7-5
Format:Whole IgG
亚型:Mouse IgG1
SDS-PAGE:25.6 kDa (Light Chain) & 52.1 kDa (Heavy Chain), under reducing conditions; 174.5 kDa, under non-reducing conditions.
纯化方法:Affinity chromatography
配对抗体:[Ab008408] Monoclonal S100B Antibody as Detection or Capture antibody in ELISA, CLIA, LF, GICA, FIA
物理外观:Purified polyclonal antibodies Antibody
储存缓冲液:10mM PBS, pH7.4
防腐剂:No
浓度:见COA
储存温度:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:37°C 下短期保存(7 天) 4°C 下短期保存(30 天)。在 -20±5°C 下长期储存(36 个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:小鼠(Mouse)
种属反应性:人(Human)
克隆号:22G7-5
偶联:Unconjugated
图片

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Sandwich ELISA - Human S100B Antibody Pair (Ab008408 & Ab008407)
Sandwich ELISA - S100B protein standard curve
S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008408) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008407) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Sensitivity: 30.3 pg/mL
Range: 0.1 ng/mL - 3.2 ng/mL

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Sandwich ELISA - Human S100B Antibody Pair (Ab008407 & Ab008408)
Sandwich ELISA - S100B protein standard curve
S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008407) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008408) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Sensitivity: 18.8 pg/mL
Range: 0.13 ng/mL - 3.1 ng/mL

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SDS-PAGE: S100B Mouse mAb (Ab008407),
S100B Mouse mAb (Ab008407) - SDS-PAGE
3 μg/lane of S100B Mouse mAb (Ab008407) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 26.0 kDa (Light Chain) & 54.2 kDa (Heavy Chain) under reducing conditions and 178.7 kDa under non-reducing conditions.

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Relevance of detection results between aladdin Antibody Pair (Ab008408 & Ab008407) and Roche in CLIA
Relevance of detection results between aladdin Antibody Pair (Ab008408 & Ab008407) and Roche in CLIA
·Capture antibody: S100B Mouse mAb (Ab008408)
·Detection antibody: S100B Mouse mAb (Ab008407)
查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/Ab008407.html



关键字: S100B小鼠单克隆抗体 ; S100B小鼠单抗;S100 calcium-binding protein B ; S-100 protein beta chain
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