基本信息
产品名称：Recombinant Cytokeratin 18 Antibody
规格或纯度：ExactAb™, Validated, Recombinant, High performance, 0.5mg/mL
应用：WB, IF/ICC, IP, Flow Cytometry, IHC
特异性：KRT18
应用：WB,Flow,IHC,IP,IF/ICC
种属反应性：人(Human), 大鼠(Rat)
交叉反应：Cross-reactivity with other species has not been measured.
免疫原：A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 18 was used as an immunogen.
阳性对照：WB: Hela, A431, HepG2, T47D, MCF-7, A549 cell lysate; Rat Liver. IHC: Colonic adenocarcinoma tissue. Flow: HCT-116 cells. ICC/IF: HCT-116 cells. IP: Hela whole cell lysate.
偶联：Unconjugated
产品属性
抗体类型：Primary antibody
克隆类型：重组抗体
克隆号：RR693
Format：Whole IgG
亚型：Rabbit IgG
SDS-PAGE：150 kDa
纯化方法：Protein A purified
来源：CHO supernatant
物理外观：Liquid
储存缓冲液：59% 1*PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3.
防腐剂：0.01% Sodium azide
浓度：0.5mg/mL
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：在 -20°C 下可保存 2 年。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属：兔(Rabbit)
种属反应性：人(Human), 大鼠(Rat)
克隆号：RR693
偶联：Unconjugated
图片
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Western Blot: Recombinant Cytokeratin 18 Antibody (Ab098863)
Recombinant Cytokeratin 18 Antibody (Ab098863) - Western Blot
All lanes: Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 44 kDa
Exposure time: 1.0 s
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ICC/IF: Recombinant Cytokeratin 18 Antibody (Ab098863)
Recombinant Cytokeratin 18 Antibody (Ab098863) - ICC/IF
Recombinant Cytokeratin 18 Antibody (Ab098863) staining Cytokeratin 18 in HCT-116 cells by IF/ICC . Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1/2000) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An AF488- conjugated Goat Anti-Rabbit IgG (1/500).
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IHC: Recombinant Cytokeratin 18 Antibody (Ab098863)
Recombinant Cytokeratin 18 Antibody (Ab098863) - IHC
Immunohistochemistry analysis of colonic adenocarcinoma tissue labelling Cytokeratin 18 with Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
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Flow Cytometry: Recombinant Cytokeratin 18 Antibody (Ab098863)
Recombinant Cytokeratin 18 Antibody (Ab098863) - Flow Cytometry
Overlay histogram showing HCT-116 cells stained Cytokeratin 18 with Recombinant Cytokeratin 18 Antibody (Ab098863) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (Ab098863, 1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
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IP: Recombinant Cytokeratin 18 Antibody (Ab098863)
Recombinant Cytokeratin 18 Antibody (Ab098863) - IP
Cytokeratin 18 was immunoprecipitated from 0.4mg of HeLa whole cell lysate with Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/25 dilution.
Lane 1: Ab098863 IP in HeLa whole cell lysate
Lane 2: PBS instead of Ab098863 in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input)
Secondary: GAR HRP for IP 1:500
Exposure time: 20.0 s
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab098863.html
关键字： Cytokeratin 18抗体 ; Cytokeratin 18兔抗体 ; CK18单克隆抗体 ; 重组CK18抗体
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