基本信息
产品名称：Recombinant ERK1 Antibody
规格或纯度：ExactAb™, Validated, Recombinant, High performance, 2mg/mL
应用：WB, IF/ICC, IP, Flow Cytometry, IHC
特异性：MAPK3
应用：WB,Flow,IF,ICC,IHC,IP
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
交叉反应：Cross-reactivity with other species has not been measured.
免疫原：A synthetic peptide corresponding to the N-term of ERK1 was used as an immunogen.
阳性对照：WB: HeLa, A431, NIH/3T3, Jurkat and K562 cell lysates. IHC: Cervix cancer tissue. Flow: HeLa cells. ICC/IF: HeLa cells. IP: A375 whole cell lysate.
偶联：Unconjugated
产品属性
抗体类型：Primary antibody
克隆类型：重组抗体
克隆号：RR671
Format：Whole IgG
亚型：Rabbit IgG
SDS-PAGE：150 kDa
纯化方法：Protein A purified
来源：CHO supernatant
物理外观：Liquid
储存缓冲液：1*PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%, pH7.3.
防腐剂：0.01% Sodium azide
浓度：2mg/mL
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：在 -20°C 下可保存 2 年。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属：兔(Rabbit)
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
克隆号：RR671
偶联：Unconjugated
图片
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Western Blot: Recombinant ERK1 Antibody (Ab102223)
Recombinant ERK1 Antibody (Ab102223) - Western Blot
All lanes: Recombinant ERK1 Antibody (Ab102223) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 43 kDa
Observed band size: 40 kDa
Exposure time: 27.0 s
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IF/ICC: Recombinant ERK1 Antibody (Ab102223)
Recombinant ERK1 Antibody (Ab102223) - IF/ICC
Recombinant ERK1 Antibody (Ab102223) staining ERK1 in HeLa cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant ERK1 Antibody (Ab102223) (1/50) at 4°C. An AF488-conjugatedGoat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An AF488-conjugatedGoat Anti-Rabbit IgG (1/500).
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Flow Cytometry: Recombinant ERK1 Antibody (Ab102223)
Recombinant ERK1 Antibody (Ab102223) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant ERK1 Antibody (Ab102223) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant ERK1 Antibody (Ab102223) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
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IP: Recombinant ERK1 Antibody (Ab102223)
Recombinant ERK1 Antibody (Ab102223) - IP
ERK1 was immunoprecipitated from 0.4 mg of A375 whole cell lysate with Recombinant ERK1 Antibody (Ab102223) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant ERK1 Antibody (Ab102223) IP in A375 whole cell lysate
Lane 2: PBS instead of Recombinant ERK1 Antibody (Ab102223) in A375 whole cell lysate
Lane 3: A375 whole cell lysate, 10 μg (input)
Exposure: 120.0 s.
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IHC: Recombinant ERK1 Antibody (Ab102223)
Recombinant ERK1 Antibody (Ab102223) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded
sections) analysis Cervix cancer tissue labelling ERK1 with Recombinant ERK1 Antibody (Ab102223) at 1:200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab102223.html
关键字： ERK1抗体 ; ERK1兔抗体 ; ERK1单克隆抗体 ; 重组ERK1抗体
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