基本信息
产品名称:GNB1 Antibody
规格或纯度:ExactAb™, Validated, Carrier Free, 0.2 mg/mL
应用:WB, IF/ICC, IHC
特异性:GNB1
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原:Fusion protein of human GNB1 (AA 100-340).
阳性对照:WB: Hep G2, A549, HEK293, Jurkat, HeLa, Raji, NIH/3T3 and C6 cell lysates. IHC: Human liver cancer tissue. IF/ICC: HeLa cells
偶联:Unconjugated
产品属性
克隆类型:多克隆抗体
Format:Whole IgG
亚型:Rabbit IgG
SDS-PAGE:150 kDa
纯化方法:Protein A purified
物理外观:Liquid
储存缓冲液:10mM PBS, 40% Glycerol, 0.05% Sodium azide, pH 7.4
防腐剂:0.05% Sodium azide
浓度:0.2 mg/mL
储存温度:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:兔(Rabbit)
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
偶联:Unconjugated
图片

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Western Blot: GNB1 Antibody (Ab105938)
GNB1 Antibody (Ab105938) - Western Blot
All lanes: GNB1 Antibody (Ab105938) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa
Exposure time: 60.0 s

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IHC: GNB1 Antibody (Ab105938)
GNB1 Antibody (Ab105938) - IHC
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using GNB1 Antibody (Ab105938). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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IF/ICC: GNB1 Antibody (Ab105938)
GNB1 Antibody (Ab105938) - IF/ICC
IF analysis of GNB1 Antibody (Ab105938) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with GNB1 Antibody (Ab105938) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/Ab105938.html



关键字: G蛋白/鸟苷酸结合蛋白抗体 ; GNB1抗体 ; GNB1多抗 ; GNB1抗体
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