基本信息
产品名称：Recombinant HDAC1 Antibody
规格或纯度：ExactAb™, Validated, Recombinant, High performance, 0.125 mg/mL
应用：WB, IF/ICC, Flow Cytometry, IHC
特异性：HDAC1
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat), 牛(Cow), 绿猴(Green Monkey)
交叉反应：Cross-reactivity with other species has not been measured.
免疫原：A synthetic peptide corresponding to the C-term of HDAC1 was used as an immunogen.
阳性对照：WB: Jurkat, K562, MOLT-4, HeLa, THP-1, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human colon tissue. Flow: HeLa cells. IF/ICC: HeLa cells.
偶联：Unconjugated
产品属性
克隆类型：重组抗体
克隆号：RR672
Format：Whole IgG
亚型：Rabbit IgG
SDS-PAGE：150 kDa
纯化方法：Protein A purified
物理外观：Liquid
储存缓冲液：59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3
防腐剂：0.01% Sodium azide
浓度：0.125 mg/mL
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属：兔(Rabbit)
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat), 牛(Cow), 绿猴(Green Monkey)
克隆号：RR672
偶联：Unconjugated
图片
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Western Blot: Recombinant HDAC1 Antibody (Ab107012)
Recombinant HDAC1 Antibody (Ab107012) - Western Blot
All lanes: Recombinant HDAC1 Antibody (Ab107012) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 61 kDa
Exposure time: 10.0 s
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Western Blot: Recombinant HDAC1 Antibody (Ab107012)
Recombinant HDAC1 Antibody (Ab107012) - Western Blot
All lanes: Recombinant HDAC1 Antibody (Ab107012) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 61 kDa
Exposure time: 19.5 s
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IF/ICC: Recombinant HDAC1 Antibody (Ab107012)
Recombinant HDAC1 Antibody (Ab107012) - IF/ICC
IF analysis of HDAC1 (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained Recombinant HDAC1 Antibody (Ab107012) at 0.25 μg/mL in blocking buffer overnight at 4 °C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
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IF/ICC: Recombinant HDAC1 Antibody (Ab107012)
Recombinant HDAC1 Antibody (Ab107012) - IF/ICC
Recombinant HDAC1 Antibody (Ab107012) staining HDAC1 in Hela cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were
fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant HDAC1 Antibody (Ab107012) (1/500) at 4°C. An AF594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An AF594-conjugated Goat Anti-Rabbit IgG (1/500).
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IHC: Recombinant HDAC1 Antibody (Ab107012)
Recombinant HDAC1 Antibody (Ab107012) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human colon tissue labelling HDAC1 with Recombinant HDAC1 Antibody (Ab107012) at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
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Flow Cytometry: Recombinant HDAC1 Antibody (Ab107012) Flow Cytometry: Recombinant HDAC1 Antibody (Ab107012)
Recombinant HDAC1 Antibody (Ab107012) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant HDAC1 Antibody (Ab107012) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant HDAC1 Antibody (Ab107012) (1/200 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab107012.html
关键字： 组蛋白去乙酰化酶1(内参)重组单克隆抗体 ; 组蛋白去乙酰化酶1重组单克隆抗体 ; 组蛋白去乙酰化酶1抗体 ; 抗HDAC1抗体
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