基本信息
产品名称：Recombinant PKC alpha Antibody
规格或纯度：ExactAb™, Validated, 重组, 0.2 mg/mL
应用：WB, IF/ICC, IHC
特异性：PRKCA
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原：A synthetic peptide derived from human PKC alpha (AA 658-672).
阳性对照：WB: K562, C6, NIH/3T3, HeLa and HEK293 cell lysates. IHC: Human colon cancer tissues. IF/ICC: HeLa cells.
偶联：Unconjugated
产品属性
克隆类型：重组抗体
Format：Whole IgG
亚型：Rabbit IgG
SDS-PAGE：150 kDa
纯化方法：Immunogen affinity purified
物理外观：Liquid
储存缓冲液：50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4
防腐剂：0.01% Sodium azide
浓度：0.2 mg/mL
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属：兔(Rabbit)
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
偶联：Unconjugated
图片
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Western Blot: Recombinant PKC alpha Antibody (Ab122024)
Recombinant PKC alpha Antibody (Ab122024) - Western Blot
All lanes: Recombinant PKC alpha Antibody (Ab122024) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa
Exposure time: 3.4 s
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IHC: Recombinant PKC alpha Antibody (Ab122024)
Recombinant PKC alpha Antibody (Ab122024) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant PKC alpha Antibody (Ab122024) at 1/50 dilution. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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IF/ICC: Recombinant PKC alpha Antibody (Ab122024)
Recombinant PKC alpha Antibody (Ab122024) - IF/ICC
IF analysis of Recombinant PKC alpha Antibody (Ab122024) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant PKC alpha Antibody (Ab122024) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab122024.html
关键字： 重组Anti- PKC alpha抗体 ; PKC alpha抗体 ; 蛋白激酶C α抗体 ; 重组 PKC alpha 抗体
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