基本信息
产品名称：Recombinant ATP1A1 Antibody
规格或纯度：ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL
应用：WB, IP, Flow Cytometry, IHC
特异性：ATP1A1
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
交叉反应：Cross-reactivity with other species has not been measured.
免疫原：A synthetic peptide corresponding to residues on the N-terminus of human NaK ATPase was used as an immunogen.
阳性对照：WB: C6, HT29, A431, Hep G2, and Raw264.7 cell lysates. IHC: Endometrium tissue. Flow: HeLa cells. IP: TT whole cell lysate.
偶联：Unconjugated
产品属性
克隆类型：重组抗体
克隆号：RR685
Format：Whole IgG
亚型：Rabbit IgG
SDS-PAGE：150 kDa
纯化方法：Protein A purified
物理外观：Liquid
储存缓冲液：59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3
防腐剂：0.01% Sodium azide
浓度：0.5 mg/mL
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属：兔(Rabbit)
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
克隆号：RR685
偶联：Unconjugated
图片
>
Western Blot: Recombinant ATP1A1 Antibody (Ab128631)
Recombinant ATP1A1 Antibody (Ab128631) - Western Blot
All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 97 kDa
Exposure time: 32.2 s
>
Western Blot: Recombinant ATP1A1 Antibody (Ab128631)
Recombinant ATP1A1 Antibody (Ab128631) - Western Blot
All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 97 kDa
Exposure time: 10.0 s
>
IHC: Recombinant ATP1A1 Antibody (Ab128631)
Recombinant ATP1A1 Antibody (Ab128631) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of endometrium tissue labelling NaK ATPase with Recombinant ATP1A1 Antibody (Ab128631) at 1/10000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
>
Flow Cytometry: Recombinant ATP1A1 Antibody (Ab128631)
Recombinant ATP1A1 Antibody (Ab128631) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant ATP1A1 Antibody (Ab128631) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant ATP1A1 Antibody (Ab128631) (1/200 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
>
IP: Recombinant ATP1A1 Antibody (Ab128631)
Recombinant ATP1A1 Antibody (Ab128631) - IP
NaK ATPase was immunoprecipitated from 0.4 mg of TT whole cell lysate with Recombinant ATP1A1 Antibody (Ab128631) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant ATP1A1 Antibody (Ab128631) IP in TT whole cell lysate
Lane 2: PBS instead of Recombinant ATP1A1 Antibody (Ab128631) in TT whole cell lysate
Lane 3: TT whole cell lysate, 10 μg (input)
Exposure: 120 s.
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab128631.html
关键字： 钠钾ATP酶蛋白a1(内参) 重组单克隆抗体 ; 钠钾ATP酶蛋白a1重组单克隆抗体 ; 钠钾ATP酶蛋白a1抗体 ; 重组ATP1A1抗体
上海阿拉丁生化科技股份有限公司是A股上市公司（（股票代码：688179），专注于科研试剂的研发、生产和销售，已陆续建立多个工厂和研发中心。作为领军企业，阿拉丁始终坚持质量第一，连续13年被评为“最受欢迎试剂品牌”。
阿拉丁目前常备库存试剂产品品种超过7万种，SKU总数超过46万，产品线涵盖了化学试剂、生化试剂、药靶配体、蛋白质和抗体等多个领域，是国内少数化学试剂到生物试剂全面发展的国产试剂品牌，产品同步发布在我们国内外电商平台。