基本信息
产品名称：Recombinant SOX10 Antibody
规格或纯度：重组, ExactAb™, 已验证, 高性能, 见COA
应用：WB, IF/ICC, IP, Flow Cytometry, IHC
特异性：SOX10
种属反应性：人(Human), 大鼠(Rat)
交叉反应：Cross-reactivity with other species has not been measured.
免疫原：A synthetic peptide derived from human SOX10 (AA 400-500).
阳性对照：WB: A375 and C6 cell lysates. IHC: Human melanoma tissue. Flow: A375 cells. ICC/IF: A375 cells. IP: A375 whole cell lysate
偶联：Unconjugated
产品属性
克隆类型：重组抗体
克隆号：RR643
Format：Whole IgG
亚型：Rabbit IgG
SDS-PAGE：150 kDa
纯化方法：Protein A purified
物理外观：Liquid
储存缓冲液：59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3
防腐剂：0.01% Sodium azide
浓度：见COA
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：长期储存-20℃（24个月）；收货后建议分装，避免反复冻融。
功能和特点
宿主种属：兔(Rabbit)
种属反应性：人(Human), 大鼠(Rat)
克隆号：RR643
偶联：Unconjugated
图片
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Western Blot: Recombinant SOX10 Antibody (Ab128734)
Recombinant SOX10 Antibody (Ab128734) - Western Blot
All lanes: Recombinant SOX10 Antibody (Ab128734) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 57, 70 kDa
Exposure time: 1.1 s
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IF/ICC: Recombinant SOX10 Antibody (Ab128734)
Recombinant SOX10 Antibody (Ab128734) - IF/ICC
Recombinant SOX10 Antibody (Ab128734) staining SOX10 in A375 cells by IF/ICC. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant SOX10 Antibody (Ab128734) (1/10000) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An AF488-conjugated Goat Anti-Rabbit IgG (1/500).
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IHC: Recombinant SOX10 Antibody (Ab128734)
Recombinant SOX10 Antibody (
Ab128734
) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labelling SOX10 with Recombinant SOX10 Antibody (Ab128734) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
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IP: Recombinant SOX10 Antibody (Ab128734)
Recombinant SOX10 Antibody (Ab128734) - IP
SOX10 was immunoprecipitated from 0.4 mg of A375 cell lysate with Recombinant SOX10 Antibody (Ab128734) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant SOX10 Antibody (Ab128734) IP in A375 whole cell lysate
Lane 2: Rabbit IgG instead of Recombinant SOX10 Antibody (Ab128734) in A375 whole cell lysate
Lane3: A375 whole cell lysate, 10 μg (input)
Exposure: 120.0 s.
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Flow Cytometry: Recombinant SOX10 Antibody (Ab128734)
Recombinant SOX10 Antibody (Ab128734) - Flow Cytometry
Overlay histogram showing A375 cells stained with Recombinant SOX10 Antibody (Ab128734)(blue). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant SOX10 Antibody (Ab128734) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (red) was used as a control.
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab128734.html
关键字： 转录因子转录因子SOX10抗体 ; 转录因子转录因子SOX10单克隆抗体 ; 转录因子转录因子SOX10重组单克隆抗体 ; SOX10抗体
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