基本信息
产品名称：Donkey Anti-Rabbit IgG H&L (AF647)
规格或纯度：ExactAb™, 已验证, 无叠氮钠, Ex:650nm, Em:668nm, 2.0mg/ml
应用：IF/ICC, Flow Cytometry
种属反应性：兔(Rabbit)
免疫原：Native rabbit IgG
阳性对照：IF/ICC: Mouse cerebellum; Mouse brain; Human brain; Rat cerebellum; Rat brain
偶联：AF647
产品属性
克隆类型：多克隆抗体
Format：Whole IgG
亚型：IgG
纯化方法：Protein G purified
物理外观：Liquid
储存缓冲液：10 mM TBS (pH=7.4) with 1% BSA, 0.03% ProClin300 and 50% Glycerol
防腐剂：0.03% ProClin300
浓度：2.0mg/ml
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：在-20℃下可保存一年。避免反复冻融循环
功能和特点
宿主种属：驴(Donkey)
种属反应性：兔(Rabbit)
偶联：AF647
图片
>
IF: Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882)
Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) - IF
IF analysis of HeLa cells labeling FXR1 with purified Recombinant FXR1 Antibody (Ab104281) at 1/1000 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution - Goat Anti-Mouse IgG H&L (AF488) 1/1000. Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) was used as the secondary antibody at 1/1000 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
>
IHC: Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882)
Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) - IHC
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min.Blocking buffer (normal goat serum) at 37°C for 30min. Antibody incubation with (GFAP) polyclonal antibody at 1/200 overnight at 4°C, followed by a conjugated Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) for 90 minutes, and DAPI for nuclei staining.
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab136882.html
关键字： Donkey Anti-Rabbit IgG H&L / AF647 ; Immunoglobulin G ; Donkey Anti-Rabbit IgG ; Donkey Anti Rabbit IgG
上海阿拉丁生化科技股份有限公司是A股上市公司（（股票代码：688179），专注于科研试剂的研发、生产和销售，已陆续建立多个工厂和研发中心。作为领军企业，阿拉丁始终坚持质量第一，连续13年被评为“最受欢迎试剂品牌”。
阿拉丁目前常备库存试剂产品品种超过7万种，SKU总数超过46万，产品线涵盖了化学试剂、生化试剂、药靶配体、蛋白质和抗体等多个领域，是国内少数化学试剂到生物试剂全面发展的国产试剂品牌，产品同步发布在我们国内外电商平台。