中文名：pNPP
英文名：pNPP
纯度：Ready-to-use (RTU)
货号：P494193
存储温度：2-8°C储存,避光
运输条件：低温运输
产品介绍：
Product Characteristics
pNPP (p-nitrophenylphosphate) is a chromogenic substrate for Alkaline Phosphatase (AP). In the presence of AP, pNPP is hydrolyzed rapidly to pnitrophenol and inorganic phosphate. Liberated pnitrophenol is seen as a clear yellow color that is measured at 405/620 nm.
pNPP, liquid ready-to-use substrate, is highly active compared to pNPP ONE (cat. No. P494181). The substrate gives superior results due to its sensitivity, allowing very low detection limits.
Composition & Properties
pNPP is a liquid, ready-to-use and aqueous formulation. The buffer solution contains stabilized pNPP and appropriate stabilizing components. Recommended stop solution is an equal volume of 0.1 M sodium hydroxide solution.
Working Procedure
1.The desired amount of substrate is poured into a sealed container and allowed to reach room temperature in the dark.
2.Recommended volumes are 100 µl substrate solution per microtiter well. Development time is typically 15- 30 minutes. Optimal time is determined by the user and will vary according to the procedure and the incubation temperature.
3.100 µl of 0.1 M NaOH is then added and mixed thoroughly.
4.In kinetic assays, the color is read directly at 405 nm. For endpoint assays the absorbance is read at 405/620 nm.
Tips & Tricks
• For use in hot climate countries, due to superior background characteristics, we recommend pNPP ONE (cat. no. P494181)
• A stop solution of 0.1 M NaOH requires no hazardous labelling. In case a higher assay sensitivity is required, a 1.0 M NaOH may be used as stop solution offering an approx. increase of OD of 3%. In both cases a 1 hour stop stability is guaranteed.
Handling & Storage
• Store solution at 2-8 °C in the dark.
• Avoid exposure to light and heat.
• Re-dispense only into bottles made of High Density Polyethylene (HDPE), amber color.
Dispensing guidelines are available upon request.
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/P494193.html
关键字： pNPP;pNPP
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