基本描述
产品名称:Recombinant Human GDNF Protein
规格或纯度:ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE)
应用:SDS-PAGE, Cell Culture, Functional Studies
生物活性:1. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is typically 3-20 ng/mL in the presence of Recombinant Human GFR alpha-1/GDNF R alpha-1 His Chimera. 2. Immobilized Recombinant Human GDNF Protein at 2μg/mL (100 μL/well) can bind Recombinant Human GFRA1 / GFRα1/GDNFRA Protein (His Tag) , the EC50 is 15-48 ng/mL.
纯度:≥97% (SDS-PAGE)
氨基酸:83-185 aa
无载体:Yes
无动物源:Yes
Accession #:P39905-2
预测分子量:15.1 KDa
SDS-PAGE:15.6 kDa, under reducing conditions; 32.6 kDa, under non-reducing conditions.
级别:无动物源, 无载体, 生物活性, ActiBioPure™, 无叠氮钠, 高性能
产品规格参数
储存缓冲液: Lyophilized from sterile PBS, 5% Trehalose, 5% Mannitol, 0.01% Tween 80, pH 7.4
复溶:It is recommended that sterile water be added to the vial to prepare astock solution of 0.25mg/mL.
储存温度:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:样品自收到之日起在 -20℃ 至 -80℃ 温度条件下可稳定保存 12 个月。收货后建议分装。避免冷冻/解冻循环。
功能和特点
Accession #:P39905-2
生物活性:1. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is typically 3-20 ng/mL in the presence of Recombinant Human GFR alpha-1/GDNF R alpha-1 His Chimera. 2. Immobilized Recombinant Human GDNF Protein at 2μg/mL (100 μL/well) can bind Recombinant Human GFRA1 / GFRα1/GDNFRA Protein (His Tag) , the EC50 is 15-48 ng/mL.
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Protein Bioactivity: Recombinant Human GDNF Protein (rp146397)
Recombinant Human GDNF Protein (rp146397) - Protein Bioactivity
Immobilized Recombinant Human GDNF Protein (rp146397) at 2 μg/mL can bind biotinylated Recombinant Human GFRA1/GFRα1/GDNFRA Protein, the EC₅₀ is 15.0 - 48.0 ng/mL.

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SDS-PAGE: Recombinant Human GDNF Protein (rp146397)
Recombinant Human GDNF Protein (rp146397) - SDS-PAGE
3 μg/lane of Recombinant Human GDNF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 15.6 kDa under reducing conditions and 32.6 kDa under non-reducing conditions.
查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/rp146397.html



关键字: 胶质细胞系来源神经营养因子(GDNF)重组蛋白 ; 重组人类 GDNF 蛋白;Astrocyte-derived trophic factor ; ATF
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