基本描述
产品名称:Recombinant Human IL-18 Protein
规格或纯度:无动物源, 无载体, 生物活性, ActiBioPure™, 无叠氮钠, 高性能, ≥95%(SDS-PAGE&HPLC)
应用:SDS-PAGE, Cell Culture, HPLC, Functional Studies
产品介绍:
白细胞介素-18 (IL-18)是一种属于IL-1超家族的细胞因子,由巨噬细胞和其他细胞产生。IL-18通过与IL-18受体结合发挥作用,在脂多糖(LPS)等微生物产物感染后,与IL-12一起诱导细胞介导的免疫。经IL-18刺激后,自然杀伤(NK)细胞和某些T细胞释放另一种重要的细胞因子干扰素-γ (IFN-γ)或ⅱ型干扰素,在激活巨噬细胞或其他细胞中起重要作用。该细胞因子与IL12的结合已被证明可以抑制IL-4依赖性IgE和IgG1的产生,并增强B细胞中IgG2a的产生。IL-18结合蛋白(IL18BP)可与该细胞因子发生特异性相互作用,从而负向调控其生物学活性。
生物活性:1、Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is typically 5 - 30 ng/mL. 2、Recombinant Human IL-18 Protein (rp147444) stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED50 for this effect is typically 0.27 ng/mL.
纯度:>95% (SDS-PAGE&SEC-HPLC)
氨基酸: 37-193 aa
无载体:Yes
无动物源:Yes
Accession #:Q14116
预测分子量:18.2 kDa
SDS-PAGE:15.4 kDa, under reducing conditions; 15.4 kDa, under non-reducing conditions.
级别:无动物源, 无载体, 生物活性, ActiBioPure™, 无叠氮钠, 高性能
产品规格参数
储存缓冲液:Lyophilized from sterile 1*PBS, 5% Trehalose, 5% Mannitol.
复溶:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25mg/mL.
储存温度:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:样品自收到之日起在 -20℃ 至 -80℃ 下可稳定保存 12 个月。收货后建议分装。避免冷冻/解冻循环。
功能和特点
Accession #:Q14116
生物活性:1、Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is typically 5 - 30 ng/mL. 2、Recombinant Human IL-18 Protein (rp147444) stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED50 for this effect is typically 0.27 ng/mL.
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Protein Bioactivity: Recombinant Human IL-18 Protein (rp147444)
Recombinant Human IL-18 Protein (rp147444) - Protein Bioactivity
Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED₅₀ for this effect is typically 5 - 30 ng/mL.

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Protein Bioactivity: Recombinant Human IL-18 Protein (rp147444)
Recombinant Human IL-18 Protein (rp147444) - Protein Bioactivity
Recombinant Human IL-18 Protein (rp147444) stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED₅₀ for this effect is typically 0.27 ng/mL.

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SDS-PAGE: Recombinant Human IL-18 Protein (rp147444)
Recombinant Human IL-18 Protein (rp147444) - SDS-PAGE
3 μg/lane of Recombinant Human IL-18 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 15.4 kDa.
查看阿拉丁官网此产品相关对应页面:https://www.aladdin-e.com/zh_cn/rp147444.html



关键字: 重组人白介素18(IL18)蛋白 ; 重组人IL-18蛋白 ; 重组人 IL-18 蛋白;Iboctadekin
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