基本信息
产品名称：BrdU Mouse mAb
规格或纯度：无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
应用：IF/ICC, Flow Cytometry
种属反应性：不依赖种属(Species independent)
免疫原：Bromodeoxyuridine
阳性对照：Flow: Jurkat cells; ICC/IF: HeLa cells
偶联：Unconjugated
产品属性
克隆类型：单克隆抗体
克隆号：AB06/4C1
Format：Whole IgG
亚型：IgG1
轻链亚型：kappa
SDS-PAGE：150 kDa
纯化方法：Protein A purified
物理外观：Liquid
储存缓冲液：PBS, pH 7.2
防腐剂： No
浓度：见COA
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：在 +4°C 下短期储存（1-2 周）。-20°C或-80°C储存。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属：小鼠(Mouse)
种属反应性：不依赖种属(Species independent)
克隆号：AB06/4C1
偶联：Unconjugated
图片
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Flow Cytometry: BrdU Mouse mAb (Ab176205)
BrdU Mouse mAb (Ab176205) - Flow Cytometry
Dot plot showing BrdU-treated (A) and DMSO-treated (B) Jurkat cells stained with Ab176205. Cells were incubated with 10 µM BrdU for 1 hour prior to being harvested. And then fixed in 100% methanol for 20 minutes at -20℃. Once fixed, pellets were acid denatured with 2M HCl for 30 minutes at room temperature.
Cells were stained with 0.1 μg/mL BrdU Mouse mAb (Ab176205) for 1 hour at room temperature. Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as a secondary antibody at a 1/1000 dilution. Propidium Iodide was used to stain total DNA.
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Flow Cytometry: BrdU Mouse mAb (Ab176205)
BrdU Mouse mAb (Ab176205) - Flow Cytometry
Flow cytometry analysis of BrdU (red line) in Jurkat cells. The cells were treated with 10μM BrdU for 1 hour. And then fixed in 100% methanol for 20 minutes at -20℃. An acid denaturation method was used to prepare the cells before labeling with BrdU Mouse mAb (Ab176205) to detect the incorporated BrdU. Cells were then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at room temperature in the dark. Unlabeled sample (black line) was used as a negative control.
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ICC/IF: BrdU Mouse mAb (Ab176205)
BrdU Mouse mAb (Ab176205) - ICC/IF
IF analysis of HeLa cells labeling BrdU with BrdU Mouse mAb (Ab176205) at 0.5 μg/mL. The cells were fixed with 100% methanol for 5 minutes and then subjected to acid hydrolysis using 2M HCl for 30 minutes at room temperature to denature the DNA. They were then blocked with 2% BSA in PBS for 1 hour and incubated overnight at 4℃ with BrdU Mouse mAb (Ab176205) at 0.5 μg/mL.
beta Tubulin Mouse mAb (Ab155823) (FITC) (shown in green) counterstained with the cells after Goat Anti-Mouse Cy5 (Ab156257) (shown in red) incubation. Nuclear DNA was labelled with DAPI (shown in blue).
Images were taken on the confocal laser scanning microscope.
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ICC/IF: BrdU Mouse mAb (Ab176205)
BrdU Mouse mAb (Ab176205) - ICC/IF
IF analysis of HeLa cells labeling BrdU with BrdU Mouse mAb (Ab176205) at 0.5 μg/mL. The cells were fixed with 100% methanol for 5 minutes and then subjected to acid hydrolysis using 2M HCl for 30 minutes at room temperature to denature the DNA. They were then blocked with 2% BSA in PBS for 1 hour and incubated overnight at 4℃ with BrdU Mouse mAb (Ab176205) at 0.5 μg/mL.
beta Tubulin Mouse mAb (Ab155823) (FITC) (shown in green) counterstained with the cells after Goat Anti-Mouse Cy5 (Ab156257) (shown in red) incubation. Nuclear DNA was labelled with DAPI (shown in blue).
Images were taken on the confocal laser scanning microscope.
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab176205.html
关键字： BrdU 小鼠单克隆抗体 ; BrdU 小鼠单抗;Bromodeoxyuridine antibody ; BrdU antibody
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