Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein, His Tag&Tag Free
分子别名（Synonym）
Integrin alpha V beta 1,ITGAV&ITGB1
表达区间及表达系统（Source）
Human ITGAV&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52E1) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gln 21 - Asp 728 (ITGB1) (Accession # NP_002201.1 (ITGAV) & NP_002202.2 (ITGB1)).
Predicted N-terminus: Phe 31 (ITGAV) & Gln 21 (ITGB1)
蛋白结构（Molecular Characterization）
Human ITGAV&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB1, has a calculated MW of 112.9 kDa (ITGAV) and 83.7 kDa (ITGB1). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 125-150 kDa (ITGAV) and 95-110 kDa (ITGB1) respectively due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
整合素α-5/β-1是纤维蛋白原的受体。整合素α-1/β-1、α-2/β-1，α-6/β-1和α-7/β-1是lamimin的受体。整合素α-4/β-1是VCAM-1的受体。它识别VCAM-1中的序列Q-I-D-S。整合素α-9/β-1是VCAM-1、细胞触觉素和骨桥蛋白的受体。它识别细胞触觉素中的序列A-E-I-D-G-I-E-L。整合素α-V/β-1也是玻连蛋白的受体。Beta-1整合素在多种配体中识别R-G-D序列。异构体2干扰异构体1，对细胞粘附和迁移（体外）产生显性负面影响。当与α-7/β-1整合素结合时，调节细胞粘附和层粘连蛋白基质沉积。
关键字： 整合素αvβ1;Integrin αvβ1;αvβ1;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group（股票代码：301080）是成立于2010年的跨国生物科技公司，是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球，横跨亚洲、北美洲、欧洲，在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家，与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。