| 名称 | Honokiol |
| 描述 | Honokiol (NSC-293100) is the active principle of magnolia extract. It inhibits the activation of Akt and enhances the phosphorylation of ERK1/ERK2. |
| 细胞实验 | Honokiol is dissolved in DMSO. In cytotoxicity assays, 10,000 cells/well are added to 96 wells plates and incubated overnight, thereafter cells are treated with different concentrations of Honokiol dissolved in dimethylsulphoxide (DMSO). Since Honokiol is not soluble in aqueous solvents, for in vitro studies Honokiol is dissolved in DMSO. To study the possible effect of DMSO on cells, solvent (DMSO) control is used at highest concentration of <0.1%. After 72 h treatment, cells are fixed and cell viability is measured by crystal violet staining (0.05%). |
| 体外活性 | Honokiol也具有直接的抗血管生成活性,因为其阻断了由VEGF-VEGFR2相互作用引起的磷酸化和外消旋活化。Honokiol通过激活caspase 8,再激活caspase 9和3,促进CLL细胞凋亡。Honokiol在黑素瘤,肉瘤,骨髓瘤,白血病,膀胱癌,肺癌,前列腺癌,口腔鳞状细胞癌和结肠癌细胞系中显示促细胞凋亡效应。Honokiol有效诱导SVR肉瘤细胞凋亡。Honokiol抑制白细胞介素4介导的CLL细胞存活,并增强苯丁酸氮芥,氟达拉滨和克拉屈滨的细胞毒性。Honokiol以不杀死PBMC的剂量杀死复发患者的骨髓瘤细胞。Honokiol处理SVR细胞,降低MAP,akt和c-src的磷酸化。用Honokiol处理,诱导Caspase 3,7,8和9及PARP裂解。Honokiol诱导结肠癌细胞RKO凋亡。Honokiol通过调节NF-κB活化途径,增强细胞凋亡,抑制破骨细胞生成并抑制侵袭。 |
| 体内活性 | Honokiol抑制小鼠移植瘤RKO细胞生长.Honokiol高效抗裸鼠SVR肉瘤.Honokiol处理小鼠移植瘤,抑制MDA-MD-231乳腺癌细胞生长. |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | Ethanol : 26.6 mg/mL (99.88 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline : 5 mg/mL (18.77 mM), Sonication is recommended.
DMSO : 255 mg/mL (957.46 mM), Sonication is recommended.
|
| 关键字 | Protein kinase B | PKB | P-Akt | NSC-293100 | NSC293100 | MEK | Inhibitor | inhibit | Honokiol | Hepatitis C virus | HCVProtease | HCV Protease | HCV | Extracellular signal regulated kinases | ERK | Autophagy | Akt |
| 相关产品 | Urea | Naringin | Aceglutamide | Alginic acid | Cysteamine hydrochloride | Hemin | Hydroxychloroquine | Stavudine | Gluconate Calcium | Tamoxifen | Paeonol | Ethyl linoleate |
| 相关库 | 已知活性化合物库 | 古代经典名方目录分子库 | 药食同源库 | 抗病毒库 | 中药单体化合物库 | 抗病毒中药单体化合物库 | 免疫/炎症分子化合物库 | 中药抗炎分子库 | 疼痛相关化合物库 | 高通量筛选天然产物库 | 抗感染天然产物库 | 抗癌药物库 |