Total RNA Purification Kits
SKU 17200
Overview
Extract high quality & quantity total RNA including miRNA
No phenol step required; isolate all RNA in one fraction
Bind & elute all RNA irrespective of size or GC content, without bias
Very sensitive & linear down to a few cells without the need for carrier RNA
Isolate from a wide variety of specimens
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information
on Norgen's NGS services
Available in a variety of formats to suit your needs
Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily
fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and
A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all
sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol.
Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the
column binds small RNAs regardless of their GC content.
Plasma/Serum Cell-Free Circulating DNA Purification Kits
SKU 55500
Overview
Isolate all sizes of circulating DNA from plasma and serum samples
Isolate viral and bacterial DNA
Versatile plasma and serum input volumes
Concentrate circulating DNA into a flexible elution volume
Isolate inhibitor-free cell-free circulating DNA
Compatible with Streck Cell-Free DNA BCT® Tubes
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a fast, reliable and convenient spin column method for the isolation of high quality, high purity and inhibitor-free
cell-free circulating DNA (cfc-DNA) from plasma/serum sample volumes. The purified plasma/serum cfc-DNA is fully compatible
with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.
Background
Plasma/Serum cell-free circulating DNA (cfc-DNA) has the potential to provide biomarkers for certain cancers and disease states
as well as fetal DNA in maternal blood. Currently, significant advancements are being made in utilizing cfc-DNA as biomarkers
for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases. Cell-free
mitochondrial DNA (cfmtDNA) is also under investigation for its clinical significance. This cfc-DNA is usually present as short
fragments of less than 1000 bp. In addition, cell-free fetal DNA has been widely used as a non-invasive method for prenatal
diagnosis including early identification of fetal sex, genetic studies for families at high risk for inherited genetic disorders,
screening for Rhesus factor, screening for aneuploidy and identification of preeclampsia.
Plasma/Serum Cell-Free Circulating DNA Purification Micro Kit
Ideal for handling initial plasma/serum volumes ranging from 10 μL up to 200 μL. The kit is designed to isolate all sizes of
cfc-DNA from either fresh or frozen plasma/serum samples and the purified DNA is eluted into a flexible elution volume
ranging from 25 µL to 50 µL.
Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit
Ideal for handling initial plasma/serum volumes ranging from 200 μL up to 500 μL. The kit is designed to isolate all sizes of
cfc-DNA from either fresh or frozen plasma/serum samples and the purified DNA is eluted into a flexible elution volume
ranging from 25 µL to 50 µL.
Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit
Ideal for handling initial plasma/serum volumes ranging from 1 mL - 4 mL. The first column will handle the large volume
input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL
Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit
Ideal for handling initial plasma/serum volumes ranging from 5 mL- 10 mL. The first column will handle the large volume
input of bodily fluids that is followed by a concentration on a mini column for a final elution of 25 µL to 50 µL
Exosomal RNA Isolation Kit
SKU 58000
Overview
Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA
Bind and elute all RNA irrespective of size or GC content, without bias
No phenol extractions
No Proteinase K treatment
No carrier RNA
Concentrate isolated RNA into a flexible elution volume ranging from 50 µL to 100 µL
Purify high-quality RNA in 15-20 minutes
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information
on Norgen's NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin
This kit provides a fast, reliable and convenient method to isolate and concentrate exosomal RNA from previously purified
exosomes. It allows for the isolation of RNA from intact extracellular vesicles (EVs) purified from different urine or
plasma/serum sample volumes. The purification is based on Norgen’s proprietary resin.
The Exosomal RNA Isolation Kit is designed to isolate all sizes of RNA, including microRNA. Moreover, the kit allows the user
to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is of the highest integrity, and can be
used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase
protection and primer extension, and expression array assays.
Single Cell RNA Purification Kit
SKU 51800
Overview
Fast and easy processing using rapid micro spin column format
Small elution volume of 8µL ready for direct utilization in qRT-PCR reactions
Isolate total RNA including microRNA in a concentrated ready-to-use format
Isolate high quality total RNA from a variety of sources
RNA can be isolated from a single cell to 100,000 cells
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Very sensitive kit designed to work with as low as a single cell and up to 100,000 cells. The special design of the micro
spin-column allows a small elution volume of as little as 8 µL.
Rapid 15 minute method for the isolation and purification of total RNA (including miRNA) from small input amounts of cultured
animal cells, CTC, stem cells, immuno-sorted cells, and microdissected samples including laser-capture microdissection (LCM).
The purified RNA is of the highest purity and integrity, and can be used in a number of qRT-PCR and digital PCR and other
whole transcriptome applications.
cf-DNAcf-RNA Preservative Tubes
SKU 63950
Overview
Preservation and isolation of both cf-DNA and cf-RNA from a single tube
Fixative-free preservative, no cross-linking of DNA
Preserve cf-DNA/ct-DNA for 30 days at ambient temperature and for up to 8 days at 37°C
Preserve cf-RNA for 30 days at ambient temperature
Preserve Circulating Tumour Cells (CTCs) for 14 days at ambient temperature
No plasma volume loss after shipping/transportation
Prevent hemolysis allowing better separation of plasma
Prevent apoptosis of blood cells and fragmentation of genomic DNA
Produce high quality/quantity of plasma cf-DNA/ct-DNA/cf-RNA
Vacuumed to draw 8.7 mL of blood in 10 mL tubes
The procedure for plasma isolation using this kit is compliant with ISO 20186-3:2019
Norgen's cf-DNA/cf-RNA Preservative Tubes are closed, evacuated plastic tubes for the collection and the preservation of
cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and
shipping. Using these tubes in combination with Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits or
Norgen's Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free
cf-DNA/ct-DNA/cf-RNA in a very small elution volume. Plasma recovered from Norgen's cf-DNA/cf-RNA Preservative
Tubes is also compatible with any other cf-DNA and/or cf-RNA purification method. The purified cf-DNA, ct-DNA and
cf-RNA are compatible with any downstream application assays, including PCR, qPCR, rt-qPCR, methylation-sensitive PCR,
Southern Blot analysis, gene expression analysis, microarrays and NGS.
This product is for research use only and not for use in diagnostic procedures.
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