Human GAD2 / GAD65 Protein, His Tag
分子别名(Synonym)
GAD2,GAD65,Glutamate decarboxylase 2
表达区间及表达系统(Source)
Human GAD2 Protein, His Tag (GA2-H55H3) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Leu 585 (Accession # Q05329).
Predicted N-terminus: Met 1
蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 67.3 kDa. The protein migrates as 65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with glycerol as protectant.
Contact us for customized product form or formulation.
运输(Shipping)
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储(Storage)
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
The product MUST be stored at -70°C or lower upon receipt;
-70°C for 3 months under sterile conditions.

背景(Background)
谷氨酸脱羧酶(GAD)是一种催化谷氨酸脱羧为γ氨基丁酸(GABA)的酶,γ氨基丁酸是高级脑区的主要抑制性递质,也是胰岛中推测的旁分泌激素。GAD使用磷酸吡哆醛(PLP)作为辅因子。GAD的两种分子形式(65 kDa和67 kDa)高度保守,均在中枢神经系统、胰岛细胞、睾丸、输卵管和卵巢中表达。这些异构体在大鼠和小鼠的大脑中呈区域性细胞质分布。GAD65是一种ampiphilic,膜锚定蛋白(585 a.a.),负责囊泡GABA的产生。GAD67是细胞质(594 a.a.),似乎负责大量的细胞质GABA产生。
关键字: GAD2;GAD2蛋白;GAD65蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group(股票代码:301080)是成立于2010年的跨国生物科技公司,是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球,横跨亚洲、北美洲、欧洲,在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家,与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。