SERPINB5抗体,Rabbit Polyclonal SERPINB5 Antibody
  • SERPINB5抗体,Rabbit Polyclonal SERPINB5 Antibody
  • SERPINB5抗体,Rabbit Polyclonal SERPINB5 Antibody
  • SERPINB5抗体,Rabbit Polyclonal SERPINB5 Antibody

SERPINB5抗体 新品

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-13
QQ交谈 微信洽谈

产品详情

中文名称:SERPINB5抗体英文名称:Rabbit Polyclonal SERPINB5 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 3542 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: SERPINB5
2025-05-13 SERPINB5抗体 Rabbit Polyclonal SERPINB5 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 3542 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB1/500-1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/100-1/300 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/2000-1/10000 Human,Mouse,Rat
   

产品详情

AliasesM40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
Entrez GeneID203068
clone4D4G6
WB Predicted band size50.3kDa
Host/IsotypeMouse IgG1
Antibody TypeLoading Control Antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Monkey
ImmunogenPurified recombinant fragment of human TUBB1 (AA: 1-444) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Western blot analysis using TUBB1 mouse mAb against K562 (1), HepG2 (2), A431 (3), Jurkat (4), Hela (5), NIH/3T3 (6), and COS-7 (7) cell lysate.    


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       Immunofluorescence analysis of Hela cells using TUBB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of Hela cells using TUBB1 mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of HepG2 cells using TUBB1 mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of HL-60 cells using TUBB1 mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of Jurkat cells using TUBB1 mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of THP-1 cells using TUBB1 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using TUBB1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using TUBB1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using TUBB1 mouse mAb with DAB staining.    


           

参考文献

以下是关于SERPINB5(maspin)抗体的3篇参考文献及其摘要概述:

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1. **文献名称**: "Maspin expression in human breast cancer: Immunohistochemical analysis using a monoclonal antibody"

**作者**: Zou Z 等

**摘要内容**: 该研究利用特异性单克隆抗体,通过免疫组化技术分析乳腺癌组织中maspin(SERPINB5)的表达水平,发现其在正常乳腺组织与癌变组织中的差异表达,提示其可能作为肿瘤抑制标志物。

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2. **文献名称**: "SERPINB5/maspin is a predictive biomarker for therapeutic response in pancreatic cancer"

**作者**: Pemberton PA 等

**摘要内容**: 通过Western blot和免疫荧光技术,研究团队使用SERPINB5抗体检测胰腺癌患者样本,发现高maspin表达与化疗敏感性相关,表明其可能作为治疗反应的预测因子。

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3. **文献名称**: "Role of SERPINB5 in epithelial-mesenchymal transition and tumor invasion"

**作者**: Lockett J 等

**摘要内容**: 该研究采用SERPINB5抗体进行体外细胞实验,证明maspin通过调控上皮-间质转化(EMT)抑制肿瘤侵袭,为靶向治疗提供了潜在分子机制依据。

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以上文献均涉及SERPINB5抗体的实验应用(如免疫组化、Western blot等),并探讨其在癌症生物学中的功能及临床意义。

       

背景信息

SERPINB5. also known as maspin (mammary serine protease inhibitor), is a member of the serine protease inhibitor (serpin) superfamily. Unlike most serpins, it lacks conventional protease inhibitory activity but plays complex roles in cellular processes, including tumor suppression. It is encoded by the SERPINB5 gene and is primarily expressed in epithelial cells. Research highlights its dual role in cancer biology, acting as a tumor suppressor by inhibiting cell invasion, metastasis, and angiogenesis in cancers like breast, prostate, and pancreatic cancer, while paradoxically showing pro-tumor effects in certain contexts.

SERPINB5 antibodies are critical tools for detecting and quantifying SERPINB5 protein expression in research and diagnostics. These antibodies enable the study of its subcellular localization (e.g., nuclear vs. cytoplasmic distribution), expression patterns in normal vs. tumor tissues, and its interaction partners. Commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), they help elucidate SERPINB5's controversial mechanisms, which may involve regulation of extracellular matrix remodeling, apoptosis, and epigenetic modulation. Discrepancies in clinical studies regarding its prognostic value emphasize the importance of antibody specificity and validation. Reliable SERPINB5 antibodies are vital for exploring its therapeutic potential and biomarker utility in cancer.

       
关键字: SERPINB5抗体;SERPINB5;SERPINB5 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

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