ACOX1抗体

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     The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using P07751(ACOX1 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P07751(ACOX1 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

The ACOX1 (Acyl-CoA Oxidase 1) antibody is a crucial tool for studying the peroxisomal β-oxidation pathway, which metabolizes very-long-chain fatty acids (VLCFAs) and branched-chain fatty acids. ACOX1. the rate-limiting enzyme in this pathway, catalyzes the first step of fatty acid oxidation, generating hydrogen peroxide and enoyl-CoA. Dysregulation of ACOX1 is linked to metabolic disorders, including peroxisomal biogenesis defects and X-linked adrenoleukodystrophy (X-ALD), as well as associations with inflammation, neurodegeneration, and cancer.

ACOX1 antibodies are widely used in research to detect the ~70-75 kDa protein in techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help investigate tissue-specific _expression (e.g., high levels in liver and kidney), subcellular localization (peroxisomes), and functional roles in lipid homeostasis. Commercially available ACOX1 antibodies are typically raised in rabbits or mice, targeting specific epitopes within its N-terminal dehydrogenase or C-terminal thioesterase domains.

Validation of antibody specificity is critical, often confirmed via knockout controls or siRNA knockdown. Researchers also utilize ACOX1 antibodies to explore its interactions with regulatory proteins (e.g., PPARα) or its role in diseases like non-alcoholic fatty liver disease (NAFLD) and cancers with altered lipid metabolism. Proper sample preparation (e.g., avoiding over-fixation) and antibody dilution optimization are essential for reliable results.